Detailed information of TA system TA170375

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 241605..242274
• Replicon: chromosome
• Accession: NZ_CP060440
• Organism: Serratia marcescens strain M158-1-1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H3V66_RS01120
• Protein ID: WP_174136732.1
• Coordinates: 241852..242274 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A8B4GS04
• Locus tag: H3V66_RS01115
• Protein ID: WP_019455657.1
• Coordinates: 241605..241871 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V66_RS01090	237182..238108	+	927	WP_240023563.1	ribokinase	-
H3V66_RS01095	238141..238749	-	609	WP_240023565.1	HD domain-containing protein	-
H3V66_RS01100	238933..239607	+	675	WP_038871224.1	hemolysin III family protein	-
H3V66_RS01105	239756..240253	+	498	WP_049199221.1	DUF2165 domain-containing protein	-
H3V66_RS01110	240292..241284	-	993	WP_049273460.1	tRNA-modifying protein YgfZ	-
H3V66_RS01115	241605..241871	+	267	WP_019455657.1	FAD assembly factor SdhE	Antitoxin
H3V66_RS01120	241852..242274	+	423	WP_174136732.1	protein YgfX	Toxin
H3V66_RS01125	242334..242852	-	519	WP_038871234.1	flavodoxin FldB	-
H3V66_RS01130	242959..243858	+	900	WP_060441367.1	site-specific tyrosine recombinase XerD	-
H3V66_RS01135	243885..244601	+	717	WP_038871236.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
H3V66_RS01140	244608..246341	+	1734	WP_240023566.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16484.52 Da
• Isoelectric Point: 10.8114

>T170375 WP_174136732.1 NZ_CP060440:241852-242274 [Serratia marcescens]
VAQWRCDIRISWRTQLFSLLTHGVLILLILISPWPEGLGPLWLVLLTLVVFQCIRSQKRIAAVQGELRLLADRRFSWHGR
EWRLAKKPWMPGYGMLLTLQPTEGKKRRRLWLASDCMSKEEWRHLRQLLLYPSTGDGEPE

Nucleotide

• Length: 423 bp

>T170375 NZ_CP077305:4721-5008 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCGTTGAGGATCTCAATCCAAGACAGACATACCGATGGCGAAGAACGTTGGCAGACCATTGGGA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAAGCAGAGCGAGATAAATATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10417.91 Da
• Isoelectric Point: 4.7921

>AT170375 WP_019455657.1 NZ_CP060440:241605-241871 [Serratia marcescens]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLNDADKALFIRLLECDDPDLFNWLMNHGAPQDSELQRMVTLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT170375 NZ_CP077305:4995-5309 [Acinetobacter pittii]
ATGAGCATGGTTAAATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAGGGAACAGTTTCTGACGATCATCTAGATCTATCAGATATGCCTGAAATTACCGACTGGAGTAATGCTGTACGCC
ACAATCAATTCTATCGTCCAGTGAAGCAACAAACATCTATTCGTTTAGATGCTGACGTACTTGCATGGTTCAAAGCACAA
GGCAAAGGTTATCAGACACGTATGAATGAAATCTTACGGGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GS04