Detailed information of TA system TA170371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 42930..43507
• Replicon: plasmid pKOX
• Accession: NZ_CP060422
• Organism: Klebsiella pneumoniae strain BA2105

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HBJ34_RS27165
• Protein ID: WP_023286013.1
• Coordinates: 43175..43507 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HBJ34_RS27160
• Protein ID: WP_023286014.1
• Coordinates: 42930..43175 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ34_RS27120	38057..38287	+	231	WP_166702400.1	hypothetical protein	-
HBJ34_RS27125	38290..38952	+	663	WP_045268437.1	hypothetical protein	-
HBJ34_RS27130	38952..39389	+	438	WP_045268438.1	hypothetical protein	-
HBJ34_RS27135	39386..40072	+	687	WP_052684046.1	hypothetical protein	-
HBJ34_RS27140	40464..40721	+	258	WP_045268511.1	hypothetical protein	-
HBJ34_RS27145	41193..41879	+	687	WP_166702398.1	hypothetical protein	-
HBJ34_RS27150	41947..42222	+	276	WP_045268439.1	hypothetical protein	-
HBJ34_RS27155	42292..42849	+	558	WP_045268440.1	hypothetical protein	-
HBJ34_RS27160	42930..43175	+	246	WP_023286014.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HBJ34_RS27165	43175..43507	+	333	WP_023286013.1	endoribonuclease MazF	Toxin
HBJ34_RS27170	43781..44101	+	321	WP_023286012.1	hypothetical protein	-
HBJ34_RS27175	44227..44865	+	639	WP_052684048.1	hypothetical protein	-
HBJ34_RS27180	44877..45179	+	303	WP_023286010.1	hypothetical protein	-
HBJ34_RS27185	45271..45690	+	420	WP_052684049.1	hypothetical protein	-
HBJ34_RS27190	45740..46054	+	315	WP_023286008.1	hypothetical protein	-
HBJ34_RS27195	46044..46250	+	207	WP_045268441.1	hypothetical protein	-
HBJ34_RS27200	46420..47280	+	861	WP_045268442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..178010	178010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11943.82 Da
• Isoelectric Point: 8.7208

>T170371 WP_023286013.1 NZ_CP060422:43175-43507 [Klebsiella pneumoniae]
MVKRYVPDAGDLIWIDFDPVAGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTQVKGYPFEVTLSGSKEGVALSDQITCV
DWRARKVTQKSTVSKSELAEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T170371 NZ_CP077303:c3475174-3474785 [Acinetobacter pittii]
TTGATAAAAGAATTAAATTTTGAGCTGAAATATAGCCGTGTCTCGGTAATATTTCAGCTTTTTGTTGGTTTGGGTTTAGC
AATCCTGCTTTATCAATTATTAACACCGATATGGTGGCTATGTGCTGTCATTCTTCTATTTATTGGTTTTATTTTCTTTC
TAAAGCAAGCACAAATATCTCAAATTGAATATTTAGATCAAAAATTATGGTCGATTGCATATTTTTCGAAAAAAGAAATT
TACCGTGGGGAAATTACTAAAATAATTGATTATCAGTTATTTGTAGTTATTTATTTTGAAGAAACTCCTACGAATATAGC
TATTGTTTGGTTCGATCAGCTTCCTATTCAGCAGTGGAAAAGATTAAAAGTTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8742.11 Da
• Isoelectric Point: 5.0176

>AT170371 WP_023286014.1 NZ_CP060422:42930-43175 [Klebsiella pneumoniae]
MAQVIVKKWGNSPSVRLPVAVMKAASLNVDDTVDIEVEDGRIIIVPARPKEYSLDALLSGISSDNLHDKVDFGPAKGKEL
L

Nucleotide

• Length: 246 bp

>AT170371 NZ_CP077303:c3475438-3475181 [Acinetobacter pittii]
ATGTCTGACGAAATGACCTTGGAAGAACGTAAAGTAATTTATCGAGCACGCCGTGGCTTAAAAGAAATTGACGTCTATTT
TGACCCGTATGTAAAAAATTATTATTTAAAAGCTGATCCAGCAGAAAAAGCATTATTTGCAGAGCTAGTTGATCAAGAAG
ATCCAGATTTATTAGATTGGTTCATGGAAGTTTCTGAGCCTCCACGTACTGAGTTACGTGAATTTATTTATAAACTAAAA
CAGTATGTGCATGGCTAA