Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489967..490604 Replicon chromosome
Accession NZ_CP060420
Organism Bacillus velezensis strain J17-4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag H8P15_RS02485 Protein ID WP_003156187.1
Coordinates 490254..490604 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag H8P15_RS02480 Protein ID WP_003156188.1
Coordinates 489967..490248 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8P15_RS02460 (H8P15_02460) 486332..486931 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
H8P15_RS02465 (H8P15_02465) 487024..487389 + 366 WP_007609580.1 holo-ACP synthase -
H8P15_RS02470 (H8P15_02470) 487554..488561 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
H8P15_RS02475 (H8P15_02475) 488678..489847 + 1170 WP_032873001.1 alanine racemase -
H8P15_RS02480 (H8P15_02480) 489967..490248 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
H8P15_RS02485 (H8P15_02485) 490254..490604 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H8P15_RS02490 (H8P15_02490) 490722..491543 + 822 WP_003156182.1 STAS domain-containing protein -
H8P15_RS02495 (H8P15_02495) 491548..491913 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
H8P15_RS02500 (H8P15_02500) 491916..492317 + 402 WP_003156178.1 anti-sigma regulatory factor -
H8P15_RS02505 (H8P15_02505) 492329..493336 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
H8P15_RS02510 (H8P15_02510) 493400..493729 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
H8P15_RS02515 (H8P15_02515) 493726..494208 + 483 WP_007609591.1 anti-sigma B factor RsbW -
H8P15_RS02520 (H8P15_02520) 494174..494962 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
H8P15_RS02525 (H8P15_02525) 494962..495564 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T170353 WP_003156187.1 NZ_CP060420:490254-490604 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T170353 NZ_CP077293:c3188555-3188452 [Klebsiella aerogenes]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT170353 WP_003156188.1 NZ_CP060420:489967-490248 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT170353 NZ_CP077293:3188444-3188590 [Klebsiella aerogenes]
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACTGTGACAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References