Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 1321181..1322097 | Replicon | chromosome |
| Accession | NZ_CP060417 | ||
| Organism | Bacillus subtilis subsp. subtilis strain ONU 559 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | - |
| Locus tag | H8S71_RS06930 | Protein ID | WP_169037464.1 |
| Coordinates | 1321351..1322097 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | G4EXD8 |
| Locus tag | H8S71_RS06925 | Protein ID | WP_003232646.1 |
| Coordinates | 1321181..1321351 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H8S71_RS06890 | 1318040..1318369 | + | 330 | WP_032725528.1 | XkdW family protein | - |
| H8S71_RS06895 | 1318366..1318530 | + | 165 | WP_014479563.1 | XkdX family protein | - |
| H8S71_RS06900 | 1318577..1319416 | + | 840 | WP_169037462.1 | phage-like element PBSX protein XepA | - |
| H8S71_RS06905 | 1319469..1319738 | + | 270 | WP_169037463.1 | hemolysin XhlA family protein | - |
| H8S71_RS06910 | 1319751..1320014 | + | 264 | WP_014479566.1 | phage holin | - |
| H8S71_RS06915 | 1320027..1320920 | + | 894 | WP_021479459.1 | N-acetylmuramoyl-L-alanine amidase | - |
| H8S71_RS06920 | 1320958..1321095 | - | 138 | WP_003232648.1 | three component toxin-antitoxin-antitoxin system antitoxin SpoIISC | - |
| H8S71_RS06925 | 1321181..1321351 | - | 171 | WP_003232646.1 | three component toxin-antitoxin-antitoxin system antitoxin SpoIISB | Antitoxin |
| H8S71_RS06930 | 1321351..1322097 | - | 747 | WP_169037464.1 | toxin-antitoxin-antitoxin system toxin SpoIISA | Toxin |
| H8S71_RS06935 | 1322207..1323208 | - | 1002 | WP_070548855.1 | inorganic phosphate transporter | - |
| H8S71_RS06940 | 1323221..1323838 | - | 618 | WP_003218470.1 | DUF47 domain-containing protein | - |
| H8S71_RS06945 | 1324114..1325430 | - | 1317 | WP_015252262.1 | serine/threonine exchanger | - |
| H8S71_RS06950 | 1325819..1326769 | + | 951 | WP_070548856.1 | ring-cleaving dioxygenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1287522..1326769 | 39247 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29047.47 Da Isoelectric Point: 4.6191
>T170352 WP_169037464.1 NZ_CP060417:c1322097-1321351 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLVPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLVPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
Download Length: 747 bp
>T170352 NZ_CP077293:c2402520-2402035 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAGCGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAGCGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6654.72 Da Isoelectric Point: 11.0203
>AT170352 WP_003232646.1 NZ_CP060417:c1321351-1321181 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
Download Length: 171 bp
>AT170352 NZ_CP077293:c2402774-2402508 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTAATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTAATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|