Detailed information of TA system TA170352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1321181..1322097
• Replicon: chromosome
• Accession: NZ_CP060417
• Organism: Bacillus subtilis subsp. subtilis strain ONU 559

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: H8S71_RS06930
• Protein ID: WP_169037464.1
• Coordinates: 1321351..1322097 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: H8S71_RS06925
• Protein ID: WP_003232646.1
• Coordinates: 1321181..1321351 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8S71_RS06890	1318040..1318369	+	330	WP_032725528.1	XkdW family protein	-
H8S71_RS06895	1318366..1318530	+	165	WP_014479563.1	XkdX family protein	-
H8S71_RS06900	1318577..1319416	+	840	WP_169037462.1	phage-like element PBSX protein XepA	-
H8S71_RS06905	1319469..1319738	+	270	WP_169037463.1	hemolysin XhlA family protein	-
H8S71_RS06910	1319751..1320014	+	264	WP_014479566.1	phage holin	-
H8S71_RS06915	1320027..1320920	+	894	WP_021479459.1	N-acetylmuramoyl-L-alanine amidase	-
H8S71_RS06920	1320958..1321095	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
H8S71_RS06925	1321181..1321351	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
H8S71_RS06930	1321351..1322097	-	747	WP_169037464.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
H8S71_RS06935	1322207..1323208	-	1002	WP_070548855.1	inorganic phosphate transporter	-
H8S71_RS06940	1323221..1323838	-	618	WP_003218470.1	DUF47 domain-containing protein	-
H8S71_RS06945	1324114..1325430	-	1317	WP_015252262.1	serine/threonine exchanger	-
H8S71_RS06950	1325819..1326769	+	951	WP_070548856.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1287522..1326769	39247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29047.47 Da
• Isoelectric Point: 4.6191

>T170352 WP_169037464.1 NZ_CP060417:c1322097-1321351 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLVPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T170352 NZ_CP077293:c2402520-2402035 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAGCGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT170352 WP_003232646.1 NZ_CP060417:c1321351-1321181 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT170352 NZ_CP077293:c2402774-2402508 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTAATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA

Structures

Antitoxin

• AlphaFold DB: G4EXD8