Detailed information of TA system TA170351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518559..519195
• Replicon: chromosome
• Accession: NZ_CP060417
• Organism: Bacillus subtilis subsp. subtilis strain ONU 559

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: H8S71_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 518845..519195 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: H8S71_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 518559..518840 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8S71_RS02600	514918..515517	-	600	WP_187002081.1	rhomboid family intramembrane serine protease	-
H8S71_RS02605	515612..515977	+	366	WP_014475874.1	holo-ACP synthase	-
H8S71_RS02610	516143..517159	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
H8S71_RS02615	517274..518443	+	1170	WP_015252766.1	alanine racemase	-
H8S71_RS02620	518559..518840	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
H8S71_RS02625	518845..519195	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
H8S71_RS02630	519310..520134	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
H8S71_RS02635	520139..520504	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
H8S71_RS02640	520508..520909	+	402	WP_003225192.1	serine/threonine-protein kinase RsbT	-
H8S71_RS02645	520921..521928	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
H8S71_RS02650	521997..522326	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
H8S71_RS02655	522323..522805	+	483	WP_187002082.1	anti-sigma B factor RsbW	-
H8S71_RS02660	522771..523559	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
H8S71_RS02665	523559..524158	+	600	WP_095252677.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T170351 WP_003156187.1 NZ_CP060417:518845-519195 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T170351 NZ_CP077293:c1113859-1113641 [Klebsiella aerogenes]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATATGAACTTTCCGACAATGAATTGGCGGTATTTTACTCAGCAGCCGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCAACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT170351 WP_003225183.1 NZ_CP060417:518559-518840 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT170351 NZ_CP077293:c1114260-1113886 [Klebsiella aerogenes]
ATGGACGAGTACTCGCCAAAAAGACATGATATCGCGCAGCTAAGATTTCTCTGCGAAACGTTGTATCATGACTGCCTTGC
AAACCTGGAAGAAAGCAACCGCGGCTGGGTTAACGATCCCACCTCGGCGGTGAATCTTCAATTAAATGAATTGATCGAGC
ACATAGCAACTTACGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATTTCTCAGCTTGACGAATACCTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAGCGATCTCCAAAAATGGCGCAAATCCGGCAATCGGTT
ATTCCGATGCTTTGTGAACGCCAGCCGGGAGAACCCGGCAAGTCTTTCTTGTTAG

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32