Detailed information of TA system TA170336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3433509..3434284
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: D3VFY6
• Locus tag: H8F46_RS15330
• Protein ID: WP_013185762.1
• Coordinates: 3433509..3434009 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: N1NS87
• Locus tag: H8F46_RS15335
• Protein ID: WP_010846171.1
• Coordinates: 3434006..3434284 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS15295	3429275..3429829	+	555	WP_038219384.1	cytochrome b	-
H8F46_RS15300	3429850..3430431	+	582	WP_010846163.1	YceI family protein	-
H8F46_RS15305	3430589..3430902	+	314	Protein_2974	type II toxin-antitoxin system RelE/ParE family toxin	-
H8F46_RS15310	3430899..3431180	+	282	WP_010846165.1	helix-turn-helix transcriptional regulator	-
H8F46_RS15315	3431485..3431748	+	264	WP_038219381.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H8F46_RS15320	3431748..3432137	+	390	WP_013185764.1	type II toxin-antitoxin system VapC family toxin	-
H8F46_RS15325	3432480..3433421	-	942	WP_013185763.1	phenylacetic acid degradation operon negative regulatory protein PaaX	-
H8F46_RS15330	3433509..3434009	-	501	WP_013185762.1	GNAT family N-acetyltransferase	Toxin
H8F46_RS15335	3434006..3434284	-	279	WP_010846171.1	DUF1778 domain-containing protein	Antitoxin
H8F46_RS15340	3434422..3435765	-	1344	WP_041977333.1	phenylacetate--CoA ligase	-
H8F46_RS15345	3435851..3437065	-	1215	WP_038219377.1	3-oxoadipyl-CoA thiolase	-
H8F46_RS15350	3437062..3437613	-	552	WP_038219375.1	hydroxyphenylacetyl-CoA thioesterase PaaI	-
H8F46_RS15355	3437606..3439234	-	1629	WP_013185758.1	3-hydroxyacyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-83)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18238.10 Da
• Isoelectric Point: 9.0299

>T170336 WP_013185762.1 NZ_CP060401:c3434009-3433509 [Xenorhabdus nematophila]
MILSAPELLSEHHYREAFNSGVTTLDQWLKNRALKNNLTGASKTYVVCSENRVLAYYSLAASAIVTDSTPGHFRRNMPNP
IPVVVLGRLAVDRSLQRKGIGRALVQDAALRIIQAADLIGVRGMIIHALSDEVKSFYETIGFEPSPLDPMLLMATLADLK
RSCTNS

Nucleotide

• Length: 501 bp

>T170336 NZ_CP077291:795190-795597 [Citrobacter farmeri]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCGCACAGTGGCTATCGCTACTCCTGCATGGGCTGGTGGCTGC
GGCTATTCTGTTAATGCCATGGCCGCTGAGTTACACGCCGCTGTGGTTGATACTGCTTTCGCTGGTGGTATTTGACTGTG
TTCGCAGCCAGCGGCGAATTAACGCCCGTCAGGGAGAGGTAAAACTTCTGATGGATGGACGGCTCCGCTGGCAGGGGCAG
GACTGGCTTATCGTCAGTGCTCCGTGGATGATCAAAACTGGCATGATGCTGCGTTTGCGTTCGGAAACCGGTAAACGCCA
GCATTTGTGGCTCGCGGCAGACAGTATGGATGAGGCAGAATGGCGGGATTTACGCCGCCTGATGATGCAAAAGTCGACGC
TGGGATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10587.22 Da
• Isoelectric Point: 6.3606

>AT170336 WP_010846171.1 NZ_CP060401:c3434284-3434006 [Xenorhabdus nematophila]
MQTPKRDTLSIRIKPETRNLIDKAAAIQGKNRTDFVLEAAQRMAEETLLEQAIMTASPEAYAKFLDRLDMPPQPNKQLRE
TMQMETPWGKEL

Nucleotide

• Length: 279 bp

>AT170336 NZ_CP077291:794943-795209 [Citrobacter farmeri]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGCCGACGCGGTATGCGTGAACTCGATATTTCCATCATGCCGTT
TTTCGAACATGAATACGACACGTTGAGCGATGATGAAAAACGTATTTTTATTCGTCTTCTGGAGTGCGATGACCCGGATT
TATTCAACTGGTTGATGAATCACGGTAAGCCGGCAGATGCTGAACTGGAGCAGATGGTACGACTCATTCAGACACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: D3VFY6

Antitoxin

• AlphaFold DB: N1NS87