Detailed information of TA system TA170332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3322843..3323487
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H8F46_RS14790
• Protein ID: WP_038220160.1
• Coordinates: 3322843..3323016 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N1NK82
• Locus tag: H8F46_RS14795
• Protein ID: WP_010847564.1
• Coordinates: 3323071..3323487 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS14770	3319171..3320553	-	1383	WP_013183087.1	envelope stress sensor histidine kinase CpxA	-
H8F46_RS14775	3320550..3321242	-	693	WP_013183086.1	envelope stress response regulator transcription factor CpxR	-
H8F46_RS14780	3321395..3321895	+	501	WP_010847568.1	Spy/CpxP family protein refolding chaperone	-
H8F46_RS14785	3322268..3322711	+	444	WP_010847567.1	hypothetical protein	-
H8F46_RS14790	3322843..3323016	+	174	WP_038220160.1	type II toxin-antitoxin system HicA family toxin	Toxin
H8F46_RS14795	3323071..3323487	+	417	WP_010847564.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H8F46_RS14800	3323595..3324843	+	1249	Protein_2877	IS3 family transposase	-
H8F46_RS14805	3325130..3325426	-	297	WP_010847563.1	helix-turn-helix domain-containing protein	-
H8F46_RS14810	3325472..3325780	-	309	WP_010847562.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H8F46_RS14815	3326126..3327025	+	900	WP_010847560.1	CDF family cation-efflux transporter FieF	-
H8F46_RS14820	3327259..3328236	+	978	WP_013183083.1	6-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6680.83 Da
• Isoelectric Point: 11.4032

>T170332 WP_038220160.1 NZ_CP060401:3322843-3323016 [Xenorhabdus nematophila]
VKQSEFRRWLEAQGVEVSNGTNHLKLKYKGRRSIMPRHPSQEIKEPLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T170332 NZ_CP077290:4151934-4152314 [Enterobacter cancerogenus]
ATGAAATGGATTAGCGCCCAGGAGGTTATTGCATTTCATGACCGTATATTGCGTTATCTTCCCGGCGTAGCCGGTATGCC
TGATGCCGGCAAAGCCGACGCCATTATCTACCGTGTCCAGAACCGCGCCCATTACGAAGGCGTAAATAACATTTTCGAAC
TGGCTGCAACGTATTGGGTTGCCATTGCTCGTGGACACATTTTTAACGACGGGAACAAGCGTACATCGTTCTTTGTCACA
ATGGCGTTTTTGCGCCGCAACGGTGTGCTGGTCTTAGACGAAGGAACAGAACTGGAAGAATTAACCGTTCAGGCCGCAAC
CGGTGAAAAAACAGTAGAGCAACTGTCAGCTATTTTGCGCACCCTGGCGATCCAATCCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15441.59 Da
• Isoelectric Point: 4.2220

>AT170332 WP_010847564.1 NZ_CP060401:3323071-3323487 [Xenorhabdus nematophila]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGETREEALEMALDALITSFEFYFEDNEKIPLPSPIGQDDDYVDVPLSITSKV
LMLNAFIDSKLTQTELASRMGVKKQEVTRIFDLRHSTKIDTVGKAAAVIGHQLTLSMQ

Nucleotide

• Length: 417 bp

>AT170332 NZ_CP077290:4151716-4151937 [Enterobacter cancerogenus]
ATGATTACCTATACATCAACGCAGGCCAGAGCCAATATCTCAGCGGTTTTAGAAAGTGCTACCAGCGGAGAGCCGGTAGA
GATTACACGGCGGGATGGTTCGGCGGCTGTCGTTATCAGCAAGGCTGAGTTTGAGGCATATCAGGCAGCGAAGCTGGATG
ATGAGTTTGATTTCATCATGCAACGGCATGGCAGAACCATCAAGGCGCTAACTGACCGATGA

Structures

Antitoxin

• AlphaFold DB: N1NK82