Detailed information of TA system TA170331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3236560..3237176
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D3VH06
• Locus tag: H8F46_RS14320
• Protein ID: WP_013183155.1
• Coordinates: 3236560..3236958 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: N1NKK1
• Locus tag: H8F46_RS14325
• Protein ID: WP_010847654.1
• Coordinates: 3236955..3237176 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS14295	3232083..3232529	-	447	WP_038219114.1	DUF805 domain-containing protein	-
H8F46_RS14300	3232588..3233208	+	621	WP_038219100.1	DUF1454 family protein	-
H8F46_RS14305	3233297..3234064	+	768	WP_038219101.1	triose-phosphate isomerase	-
H8F46_RS14310	3234580..3235356	+	777	WP_010847657.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
H8F46_RS14315	3235419..3236318	+	900	WP_041979641.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
H8F46_RS14320	3236560..3236958	-	399	WP_013183155.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H8F46_RS14325	3236955..3237176	-	222	WP_010847654.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H8F46_RS14330	3237435..3237995	-	561	Protein_2783	DUF4102 domain-containing protein	-
H8F46_RS14335	3238323..3239147	-	825	WP_038219103.1	DNA damage-inducible protein D	-
H8F46_RS14340	3239857..3240069	-	213	WP_113935543.1	ogr/Delta-like zinc finger family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3236560..3253734	17174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (5-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14931.84 Da
• Isoelectric Point: 6.3262

>T170331 WP_013183155.1 NZ_CP060401:c3236958-3236560 [Xenorhabdus nematophila]
MIWVSAQEVIAFHDRILQRLPGVAGMPDSGRAEALIYRVQNRTHHEGITDIFELAATYWAAIARGHIFNDGNKRTAFFVT
MTFLYRNGIKIRDNDNTLENLTVDAATGEKTVDQLAQHLRELVDYTNPSCNP

Nucleotide

• Length: 399 bp

>T170331 NZ_CP077290:3621508-3621918 [Enterobacter cancerogenus]
GTGGTCCTGTGGCAATCTGAACTTCGCGTCTCATGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTGGCGGC
CTTTGTACTACTGATGCCGTGGCCCCTCAGCTACACGCCTCTGTGGTTGCTGCTGCTGTCATTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGCCGGATTAATGCCCGTCAGGGTGAGATCAAACTGCTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGACATCATCGGTACGCCGTGGATGCTCAATTCCGGCATGATGCTGCGCTTGCGAAGCGTGGATGGAGAGCGTCG
CCAGCACCTGTGGCTGGCGGCTGACAGTATGGACGCTGCCGAGTGGCGGGATCTGCGCCGGACAATGTTGCAGCAGCCGA
CGCAGGATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8171.99 Da
• Isoelectric Point: 5.5589

>AT170331 WP_010847654.1 NZ_CP060401:c3237176-3236955 [Xenorhabdus nematophila]
MRTYTSTQARANIAEVLDTAAHGEPVEITRRDGSSAVVISRAEFDNWQNAKLDAEFDSIMQRHGHTIEALTNR

Nucleotide

• Length: 222 bp

>AT170331 NZ_CP077290:3621261-3621527 [Enterobacter cancerogenus]
ATGGATATTAACAACAAGGCCCGTATCCACTGGGCGTGCCGTCGCGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGACACCCTGAGCGACGACGATAAGCGGCTCTTTGTGCGCCTGCTCGAATCAGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: D3VH06

Antitoxin

• AlphaFold DB: N1NKK1