Detailed information of TA system TA170328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3033282..3033868
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H8F46_RS13250
• Protein ID: WP_038218882.1
• Coordinates: 3033282..3033611 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: N1NFW9
• Locus tag: H8F46_RS13255
• Protein ID: WP_010845238.1
• Coordinates: 3033611..3033868 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS13225	3029008..3029655	+	648	WP_038218884.1	peptide-methionine (S)-S-oxide reductase MsrA	-
H8F46_RS13230	3029741..3031072	+	1332	WP_010845233.1	hemolysin family protein	-
H8F46_RS13235	3031187..3031393	-	207	WP_010845234.1	DUF1107 domain-containing protein	-
H8F46_RS13240	3031720..3032277	+	558	WP_010845235.1	YtfJ family protein	-
H8F46_RS13245	3032369..3033112	-	744	WP_010845236.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
H8F46_RS13250	3033282..3033611	-	330	WP_038218882.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8F46_RS13255	3033611..3033868	-	258	WP_010845238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H8F46_RS13260	3034105..3034725	-	621	WP_010845240.1	peptidylprolyl isomerase	-
H8F46_RS13265	3034872..3035690	-	819	WP_038218880.1	DUF3037 domain-containing protein	-
H8F46_RS13270	3035994..3036362	-	369	WP_010845243.1	hypothetical protein	-
H8F46_RS13275	3036587..3037039	-	453	WP_010845244.1	50S ribosomal protein L9	-
H8F46_RS13280	3037078..3037305	-	228	WP_010845245.1	30S ribosomal protein S18	-
H8F46_RS13285	3037310..3037627	-	318	WP_010845246.1	primosomal replication protein N	-
H8F46_RS13290	3037633..3038031	-	399	WP_010845247.1	30S ribosomal protein S6	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• SpoVT-AbrB domain (13-47)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12270.43 Da
• Isoelectric Point: 9.5936

>T170328 WP_038218882.1 NZ_CP060401:c3033611-3033282 [Xenorhabdus nematophila]
MYIPDKGDIISLNSDPSAGKEIMKRRPAFVISRKMFNEHTGFAVVVPITSTARGMKLEVILPEEVSVQGSILIHQVKSLD
FSERQVKFIEKAPQYIIDRVTELTKAIIL

Nucleotide

• Length: 330 bp

>T170328 NZ_CP077290:c2090772-2090458 [Enterobacter cancerogenus]
ATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCCGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAACGTATCT
GCTGGATCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGCGGCCATCGAGGAGCATATCGATGCAG
GAATCACGCTCGCCGATGCTGTTAATTTTCTGGTAGAACGTTATGAACTGGTACGCATCGACCGCCAAGGGTTTAGCTGG
CAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAATAAATACTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9283.71 Da
• Isoelectric Point: 4.5959

>AT170328 WP_010845238.1 NZ_CP060401:c3033868-3033611 [Xenorhabdus nematophila]
MTAVVIRQSGGANIVSIPKAIVKTLNLHTGSKLELSIQDNKIVLTPVEEELTLESLLAGSPKECFRVTDEDREWLEANPV
GKEVI

Nucleotide

• Length: 258 bp

>AT170328 NZ_CP077290:c2091138-2090815 [Enterobacter cancerogenus]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGGAGTTTTAGCCCCATGCACCTGCAGAAATTAGATCTGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAGATATGCTGCGTGCTGGCGAACTAAATCCTCATCAGCAACGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATCCCCGTGGTAGCTGCGGTTACGTTTACATCTCTATTTATCCCACACC
GTAA

Structures

Antitoxin

• AlphaFold DB: N1NFW9