Detailed information of TA system TA170326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2593497..2594115
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: N1NUC1
• Locus tag: H8F46_RS11210
• Protein ID: WP_010845060.1
• Coordinates: 2593497..2593700 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: N1NQW2
• Locus tag: H8F46_RS11215
• Protein ID: WP_010845061.1
• Coordinates: 2593747..2594115 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS11190	2590004..2590342	+	339	WP_010845056.1	P-II family nitrogen regulator	-
H8F46_RS11195	2590357..2591640	+	1284	WP_038220148.1	ammonium transporter AmtB	-
H8F46_RS11200	2591815..2592669	-	855	WP_010845058.1	acyl-CoA thioesterase II	-
H8F46_RS11210	2593497..2593700	-	204	WP_010845060.1	hemolysin expression modulator Hha	Toxin
H8F46_RS11215	2593747..2594115	-	369	WP_010845061.1	Hha toxicity modulator TomB	Antitoxin
H8F46_RS11220	2594631..2595657	+	1027	Protein_2191	IS630 family transposase	-
H8F46_RS11225	2595884..2597132	+	1249	Protein_2192	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8077.34 Da
• Isoelectric Point: 6.9770

>T170326 WP_010845060.1 NZ_CP060401:c2593700-2593497 [Xenorhabdus nematophila]
MTKTDYLMRLRKCTSIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 204 bp

>T170326 NZ_CP077290:2047351-2047872 [Enterobacter cancerogenus]
GTGAACGATGTGAAGATAGGGATTTTTTCAGAAGACGTTGAGTACGACCTGAGCCAGTTCGATTGCGGAGAAGTAAGCCT
TAACACCTTCCTTGCAGAACACCTTAAACGCCAGCATCGCGGGAAGTTTTTGCGTGCCTACGTGCTCACGACCCGCGAGG
AAAACCCGCGAGTTCTGGGTTATTACACGCTCTCCGGGAGTTGCTTTGAAAAAGCTTACCTGCCCTCTAAAACTCAGCAG
AAGCGCATTCCTTATAAAAATGTGCCGAGCGTAACCTTAGGGCGTCTGGCTATTGATAAACGCATTCAGGGGCAAGGTTT
TGGTGAGTTACTTGTCACCCATGCTATGAAGACCGTCTATTTGGCCTCCTTTGCCGTCGGCATTCATGGTCTGTTTGTTG
AAGCGCTGAACGATACAGCCAGAAATTTTTACCTCAAACTGGGGTTCATACCGCTTCTGGCCGAGAATGAATTCACGCTC
TTCTTACCGACGAAAACCTTTGAAAGCATGTTTGAAGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14490.38 Da
• Isoelectric Point: 5.1920

>AT170326 WP_010845061.1 NZ_CP060401:c2594115-2593747 [Xenorhabdus nematophila]
MDEYSPKRYDIAELKYLCEKLIHEALSVLNRADSHWIHDLTSEKSLKLNELIEHIAAFVWNFKIKYTDHYDLSTLIDEYL
DETYNLFGSDKISFLELTKWQRTNEHLTNILLHDLNASLSKT

Nucleotide

• Length: 369 bp

>AT170326 NZ_CP077290:2047078-2047347 [Enterobacter cancerogenus]
ATGACAGCACTGAAGAAACAGCGCATCGATCTGAGGTTAAGCGAAGACGATAAAAGCCTGATCGAAGAAGCTGCAGCAAT
GACCAACCAGTCAATTTCGCAGTTCATGGTCAGCACGGCTTCTGAACGCGCTGCGGAAGTTATCGATCGACACCGTCGCA
TGATCCTTAATGAAGCCTCCTGGAATACGGTAATGGAGGCCATCAGCAACCCGCCGGCACCAAATGACCGCCTGAAACGC
GCGGCGAAGCGTCTCCAGGATATGGAGTAA

Structures

Toxin

• AlphaFold DB: N1NUC1

Antitoxin

• AlphaFold DB: N1NQW2