Detailed information of TA system TA170322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 112969..113551
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: D3VJ77
• Locus tag: H8F46_RS00550
• Protein ID: WP_013184681.1
• Coordinates: 112969..113256 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H8F46_RS00555
• Protein ID: WP_010846808.1
• Coordinates: 113267..113551 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS00525	109952..110824	+	873	WP_013185710.1	IS630 family transposase	-
H8F46_RS00530	111154..111579	-	426	WP_012987825.1	type II toxin-antitoxin system VapC family toxin	-
H8F46_RS00535	111579..111833	-	255	WP_010846804.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H8F46_RS00540	112058..112387	+	330	WP_010846805.1	type I toxin-antitoxin system SymE family toxin	-
H8F46_RS00545	112528..112866	+	339	WP_010846806.1	SymE family type I addiction module toxin	-
H8F46_RS00550	112969..113256	+	288	WP_013184681.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H8F46_RS00555	113267..113551	+	285	WP_010846808.1	putative addiction module antidote protein	Antitoxin
H8F46_RS00560	113634..114017	-	384	WP_041976221.1	hypothetical protein	-
H8F46_RS00565	114138..114347	-	210	WP_013184683.1	hypothetical protein	-
H8F46_RS00570	114470..115501	-	1032	WP_119365216.1	IS630 family transposase	-
H8F46_RS00575	115583..115780	+	198	WP_081990858.1	replication initiation protein	-
H8F46_RS00580	115830..116444	-	615	WP_010849037.1	DUF4158 domain-containing protein	-
H8F46_RS00585	116652..117257	+	606	WP_113935522.1	recombinase family protein	-
H8F46_RS00590	117333..117587	+	255	WP_052145619.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	83179..173892	90713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10773.43 Da
• Isoelectric Point: 10.0371

>T170322 WP_013184681.1 NZ_CP060401:112969-113256 [Xenorhabdus nematophila]
MITIERTQNFEKWLKSLKDRIAKAKILIRVERMGEGNFGDVESVGGGVSELRIHYGQGYRVYFTNKNNEIILLLCGGDKS
SQQADIKKAKQLAKE

Nucleotide

• Length: 288 bp

>T170322 NZ_CP077280:c86151-85666 [Klebsiella quasipneumoniae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10443.90 Da
• Isoelectric Point: 9.3655

>AT170322 WP_010846808.1 NZ_CP060401:113267-113551 [Xenorhabdus nematophila]
MKSAPKKFDVVEFLETEEDIQAYLNAAIEENDTKYLFKALGNIARRKNISQLSKESGISREGIYKALSGEGNPSFNTVYK
ISKALGLKLHFTGA

Nucleotide

• Length: 285 bp

>AT170322 NZ_CP077280:c86405-86139 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCCAAGGAATCACAGCGGGCGCTCATTGACGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAATGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: D3VJ77