Detailed information of TA system TA170316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 2044380..2045133
• Replicon: chromosome
• Accession: NZ_CP060398
• Organism: Synechococcus sp. CBW1002

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H8F24_RS09845
• Protein ID: WP_197153729.1
• Coordinates: 2044648..2045133 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: H8F24_RS09840
• Protein ID: WP_197153728.1
• Coordinates: 2044380..2044661 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F24_RS09825	2039430..2040977	-	1548	WP_197169597.1	IS1182 family transposase	-
H8F24_RS09830	2041155..2042030	-	876	WP_197153726.1	hypothetical protein	-
H8F24_RS09835	2042219..2044420	+	2202	WP_197169598.1	AAA family ATPase	-
H8F24_RS09840	2044380..2044661	+	282	WP_197153728.1	DUF1778 domain-containing protein	Antitoxin
H8F24_RS09845	2044648..2045133	+	486	WP_197153729.1	GNAT family N-acetyltransferase	Toxin
H8F24_RS09850	2045168..2045392	+	225	WP_197153731.1	hypothetical protein	-
H8F24_RS09855	2045473..2046324	+	852	WP_197169599.1	hypothetical protein	-
H8F24_RS09860	2046317..2046811	+	495	WP_197169600.1	hypothetical protein	-
H8F24_RS09865	2046880..2047425	+	546	WP_197153734.1	hypothetical protein	-
H8F24_RS09870	2047494..2047724	+	231	WP_197153735.1	hypothetical protein	-
H8F24_RS09875	2047733..2048113	+	381	WP_197153736.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H8F24_RS09880	2048128..2048325	+	198	WP_197153737.1	hypothetical protein	-
H8F24_RS09885	2048303..2048707	-	405	WP_197153738.1	DNA-binding protein	-
H8F24_RS09890	2048704..2049207	-	504	WP_197159094.1	GNAT family N-acetyltransferase	-
H8F24_RS09895	2049315..2049680	-	366	WP_197153739.1	AbrB family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2036342..2061382	25040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-89)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17337.93 Da
• Isoelectric Point: 8.7818

>T170316 WP_197153729.1 NZ_CP060398:2044648-2045133 [Synechococcus sp. CBW1002]
MRPPEPLGPQHQLEGFDCGNTALNRWLQQRALANERQGVSRTVVVCSDEDSNVIAYACLSAGAIVLAEVPGALRRNMPDP
LPVVVLGRLAVDRRHQGQGLGRALVADALRRSLAARRLIGARALLVHAIDEQAAAFYRSLGFRPSPLSASTLMLQLSEPD
G

Nucleotide

• Length: 486 bp

>T170316 NZ_CP077270:1823354-1823503 [Staphylococcus haemolyticus]
ATGACTAAAAATAAAGATTCAGAATTAAATTATCATGAGGAAGAAAATGCGATGGTTCAAGATTTAGATGACTTGAAAAC
CTTGGGTAAAGAAATGGAACAAATTTCTGAAGAAAACGACGAGGACAAGTTGAATCAATCGCATGAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10315.54 Da
• Isoelectric Point: 4.3184

>AT170316 WP_197153728.1 NZ_CP060398:2044380-2044661 [Synechococcus sp. CBW1002]
MARDQVLQLRASAEQRQLIDQAAAAEGISRTEFILRSCQERARDVLLDRTLFSLTQEQTTALLADLEDSGAAAQDQASIE
RLLDLPQPWHAPA

Nucleotide

• Length: 282 bp

>AT170316 NZ_CP077270:1822732-1823331 [Staphylococcus haemolyticus]
ATGGCAATGAACTTTAAAGTATTTAATGATGTAGAACATGTAGCTGAGTACACAGCAGATATCATTAGAAAGCAATTTAA
TAATAACCCTACAACTATTGCGGGAATTCATTTAACAAAGGATGCTGCACCTGTTTTAGATGAGTTAAAGAAAGATGTAG
ATCATAATGCGGTTGATTTTAGTCAAGTAAATATCTTAGATTATGACGATAATCGCTCTTACTATGAAGCATTAGGTGTA
CCTGCTAGTCAAATTTATCCAATTAATCTTGACGATGATGCAGAATCTTTAATTGATGACAAAATTAAAACTAAAGAAAA
TAAAGGTAAATTAATTTTACAAGTTACTTCTATTGATGAATCAGGTAGTTTAAATGTAAATGTAAGACAAGGATTATTAA
AAGCGCGTGAAGTAGTATTAGTTGTGACTGGTGCTAATAAACGTGAAGTTGTTAAAAAACTTTATGAAGAAAATGGTAAA
TCTAGCTTTGAACCATCTGATTTAAAAGCTCATAGAATGGTAACAGTTGTTTTAGATAGAGCAGCTGCAGAAGGTTTACC
TGAGGATGTTAAAGAATATTTCACTGCTAGATTTGCTTAA