Detailed information of TA system TA170289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 885857..886465
• Replicon: chromosome
• Accession: NZ_CP060396
• Organism: Synechococcus sp. CBW1006

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: H8F26_RS04180
• Protein ID: WP_197157702.1
• Coordinates: 885857..886147 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H8F26_RS04185
• Protein ID: WP_197157703.1
• Coordinates: 886157..886465 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F26_RS04155	881278..882291	-	1014	WP_197157694.1	methyltransferase	-
H8F26_RS04160	882391..883299	+	909	WP_197157696.1	MBL fold metallo-hydrolase	-
H8F26_RS04165	883321..884056	+	736	Protein_820	tyrosine-type recombinase/integrase	-
H8F26_RS04170	885263..885472	+	210	WP_197157698.1	type II toxin-antitoxin system HicB family antitoxin	-
H8F26_RS04175	885475..885702	+	228	WP_197157700.1	type II toxin-antitoxin system HicA family toxin	-
H8F26_RS04180	885857..886147	+	291	WP_197157702.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H8F26_RS04185	886157..886465	+	309	WP_197157703.1	putative addiction module antidote protein	Antitoxin
H8F26_RS04190	886601..886858	+	258	WP_197157705.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H8F26_RS04195	886852..887187	+	336	WP_197157707.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H8F26_RS04200	887350..887871	+	522	Protein_827	DUF1254 domain-containing protein	-
H8F26_RS04205	887955..888584	+	630	WP_197154053.1	ISAs1 family transposase	-
H8F26_RS04210	888581..889120	+	540	WP_197158971.1	ISAs1 family transposase	-
H8F26_RS04215	889137..890135	+	999	Protein_830	DUF1254 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	885714..888006	2292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-89)

Antitoxin

• Uncharacterized protein HI1420 (3-98)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10795.39 Da
• Isoelectric Point: 10.2313

>T170289 WP_197157702.1 NZ_CP060396:885857-886147 [Synechococcus sp. CBW1006]
MLEIRETSAYATWFSSLRDRTAKARIDIRIRRLSLGNPGDVRPVGEGVSELRIHYGPGYRIYLTMQGEAIVILLAGGDKS
SQDQDIRLAKHLARNL

Nucleotide

• Length: 291 bp

>T170289 NZ_CP077262:124052-124363 [Citrobacter pasteurii]
GTGCATGTTATCAGCCGAAAACCATTCAATGAGGGTATGCTCATGTATCCAAACCATGGGCTTGCTCTCTCTGAGTTGCT
GCATGTTTTGGAGAAAAAAACATTCCATCACCCTGAAGAGATGAAACAGTATATTCCTTCTCTTGATAATTTTAAGTACA
GGAATAAATGGTGGGTAATTGATGTTTCTGGTAACAACCTGAGACTCATTGCCTACATAGATTTCAGGTTACATAAGGTA
TTTGTGAAGCATATCGTTCTCCACGCCGAATATGACAAACTGACAGAATATTATCGGGACCATAAAGAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10549.03 Da
• Isoelectric Point: 4.9721

>AT170289 WP_197157703.1 NZ_CP060396:886157-886465 [Synechococcus sp. CBW1006]
MTKTITTIWDPAAHLTTADDVAAYLEAALQDGDPQLVAAALGDIARAKGMSQIAREAGLGRESLYKSLSSSGNPELATVL
KVISALGLQLHVSATDNAKMAV

Nucleotide

• Length: 309 bp

>AT170289 NZ_CP077262:124360-124806 [Citrobacter pasteurii]
ATGAGATCTCATCGTCATATGGACGCAGCCAGCGCAAAAAAGATCGTTGACACCTTCAGTTGTGCTGTAAAAACGATCCC
TCTTTTGGGGGAAGAACGCAATGAAACGGAGTATCGAAGGGCACTAGCGCTAGTAGAGTTTCTGGTTGACCACGACGATC
TTGAAAACCCACTATTTGAATTACTTAGTGCCCGAATCAGTGAATACGAAAAACATGCACCAGAATTCAGAGCGCTCAAT
CAACACCTGAAACAAATCCCCTCTGGCGTTTCCGTATTGCGTACGCTCATGGATCAGTACGGATTAAAAGCCACCGACCT
CGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCCTAAATGGCCGTAGAGCACTGACTGTAAGCCATATTAAAG
CGCTTGCAGAACGCTTTAAACTTCCGGCAGATGCATTTATCGAGTAG