Detailed information of TA system TA170282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2020654..2021698
• Replicon: chromosome
• Accession: NZ_CP060395
• Organism: Synechococcus sp. CBW1108

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H8F27_RS10975
• Protein ID: WP_197148121.1
• Coordinates: 2020654..2021223 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: H8F27_RS10980
• Protein ID: WP_197148122.1
• Coordinates: 2021225..2021698 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F27_RS10950	2016454..2016885	-	432	WP_197153519.1	DUF3119 family protein	-
H8F27_RS10955	2016915..2017679	-	765	WP_197148119.1	ABC transporter permease	-
H8F27_RS10960	2017676..2018968	-	1293	WP_197153520.1	MFS transporter	-
H8F27_RS10970	2019527..2020672	+	1146	WP_197148120.1	hypothetical protein	-
H8F27_RS10975	2020654..2021223	-	570	WP_197148121.1	PIN domain-containing protein	Toxin
H8F27_RS10980	2021225..2021698	-	474	WP_197148122.1	helix-turn-helix domain-containing protein	Antitoxin
H8F27_RS10985	2022032..2024317	+	2286	WP_197153521.1	DEAD/DEAH box helicase	-
H8F27_RS10990	2024384..2025337	+	954	WP_197147974.1	IS1595 family transposase	-
H8F27_RS10995	2025447..2025767	+	321	WP_197148123.1	DUF1778 domain-containing protein	-
H8F27_RS11000	2025772..2026317	+	546	WP_197148124.1	GNAT family N-acetyltransferase	-
H8F27_RS11005	2026322..2026486	-	165	WP_197148125.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2024384..2025337	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Helix-turn-helix domain, group 17 (87-131)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21568.66 Da
• Isoelectric Point: 5.5354

>T170282 WP_197148121.1 NZ_CP060395:c2021223-2020654 [Synechococcus sp. CBW1108]
VNRFTVVYDACVLYPAPLRDLLMRLALTDLYRARWSDQIHEEWITAVLRNRPDLSREQLERTRSLMNAHVRDALVDGHQS
LIPALELPDPDDRHVLAAAIKCGADLILTFNLDDFPEHVLASYGLGACHPDLFLVDQLNLDAERVCMAMRQHRASLRNPP
KSVEQYLVTLEEQGLSKFSEAVRHYAAEL

Nucleotide

• Length: 570 bp

>T170282 NZ_CP077260:c921768-921565 [Providencia rettgeri]
ATGACCAAATCTGATTATCTGATGCGTTTAAGAAAATGCACCACAATTGAAACACTTGAGCGTGTCATTGAAAAAAATAA
GTATGAATTATCTGATGATGAGCTAGAGCTATTCTATTCAGCAGCAGATCACCGCTTAGCTGAACTCACAATGAATAAGC
TTTATGATAAAATCCCAGCGTCTGTTTGGAAGTTTGTTAGATAA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 16767.18 Da
• Isoelectric Point: 4.9286

>AT170282 WP_197148122.1 NZ_CP060395:c2021698-2021225 [Synechococcus sp. CBW1108]
MAVALPSSIEPVIPTAEAAEAAGASLRDLAGFRASHGLRQASLSLDLDDGQRASVPIPAAVLELLQGILVQMAQGNAVTL
VPVHAELTTQQAADLLNVSRPFLIERLEQGEIPFRKVGTHRRIRLADLMAYKHDIDQQRGAALDELAAQAQELGMGY

Nucleotide

• Length: 474 bp

>AT170282 NZ_CP077260:c922211-921843 [Providencia rettgeri]
ATGGATGAATACTCACCCAAAAGACATGATATAGCAGAACTAAAATACTTATGTAATAGTCTCAATAGAGACGCTATTTT
AAGCTTACAAAAAACAAATACACACTGGATTAATGATTTAAGCTCCCCGCAAAGCGCAGCCTTGAATGAACTCATTGAAC
ATATCGCTGCATTTGTATGGCGGTTTAAAATCAAATACCCAAAAGAAAATCTTGTTATATCACTTATCGAAGAATACCTC
GATGAGACCTATGATTTATTTGGCAGCCCAGTTATCACACTTAGTGAAATTATAGACTGGGAAGCAATGAATCACAGCCT
TGTCGCTGTGCTGGATGATGACCTAAAGTGCCTAACCAGCAAAACTTAA