Detailed information of TA system TA170268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4776394..4777652
• Replicon: chromosome
• Accession: NZ_CP060392
• Organism: Pseudomonas aeruginosa strain 1903031130

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: H8A93_RS22225
• Protein ID: WP_003086331.1
• Coordinates: 4776394..4777149 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: H8A93_RS22230
• Protein ID: WP_003086329.1
• Coordinates: 4777284..4777652 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8A93_RS22215	4771565..4774108	+	2544	WP_003086335.1	acyl-homoserine-lactone acylase QuiP	-
H8A93_RS22220	4774263..4775624	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
H8A93_RS22225	4776394..4777149	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
H8A93_RS22230	4777284..4777652	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
H8A93_RS22235	4777725..4779011	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
H8A93_RS22240	4779067..4780656	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
H8A93_RS22245	4780735..4781211	+	477	WP_003086322.1	hypothetical protein	-
H8A93_RS22250	4781214..4781489	-	276	WP_023098713.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T170268 WP_003086331.1 NZ_CP060392:c4777149-4776394 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T170268 NZ_CP077242:1927758-1927865 [Escherichia fergusonii]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATCGCTGGCATTCTTGCCAGTTTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT170268 WP_003086329.1 NZ_CP060392:c4777652-4777284 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT170268 NZ_CP077242:c1927709-1927646 [Escherichia fergusonii]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7