Detailed information of TA system TA170267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4213278..4214320
• Replicon: chromosome
• Accession: NZ_CP060392
• Organism: Pseudomonas aeruginosa strain 1903031130

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H8A93_RS19525
• Protein ID: WP_003153636.1
• Coordinates: 4213278..4213853 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: H8A93_RS19530
• Protein ID: WP_003050245.1
• Coordinates: 4213850..4214320 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8A93_RS19500	4209716..4210441	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
H8A93_RS19505	4210480..4211382	-	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
H8A93_RS19510	4211382..4211882	-	501	WP_003090159.1	hypothetical protein	-
H8A93_RS19515	4211879..4212349	-	471	WP_063305693.1	hypothetical protein	-
H8A93_RS19520	4212346..4213260	-	915	WP_016852809.1	AAA family ATPase	-
H8A93_RS19525	4213278..4213853	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
H8A93_RS19530	4213850..4214320	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
H8A93_RS19535	4214524..4214907	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
H8A93_RS19540	4214904..4215137	+	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
H8A93_RS19545	4215154..4215513	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
H8A93_RS19550	4215525..4215923	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
H8A93_RS19555	4215920..4216612	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
H8A93_RS19560	4216609..4217520	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
H8A93_RS19565	4217510..4218928	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4164882..4288895	124013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T170267 WP_003153636.1 NZ_CP060392:c4213853-4213278 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T170267 NZ_CP077242:1927286-1927393 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
TGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT170267 WP_003050245.1 NZ_CP060392:c4214320-4213850 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT170267 NZ_CP077242:c1927237-1927174 [Escherichia fergusonii]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Antitoxin

• AlphaFold DB: I3TV68