Detailed information of TA system TA170265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1915290..1916798
• Replicon: chromosome
• Accession: NZ_CP060392
• Organism: Pseudomonas aeruginosa strain 1903031130

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H8A93_RS08880
• Protein ID: WP_073660237.1
• Coordinates: 1915290..1915877 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1C7C4U7
• Locus tag: H8A93_RS08885
• Protein ID: WP_003091607.1
• Coordinates: 1915944..1916798 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8A93_RS08875	1911545..1915024	-	3480	WP_003091610.1	hybrid sensor histidine kinase/response regulator	-
H8A93_RS08880	1915290..1915877	+	588	WP_073660237.1	GNAT family N-acetyltransferase	Toxin
H8A93_RS08885	1915944..1916798	+	855	WP_003091607.1	AraC family transcriptional regulator	Antitoxin
H8A93_RS08890	1916895..1919060	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
H8A93_RS08895	1919235..1921109	+	1875	WP_003091605.1	MFS transporter	-
H8A93_RS08900	1921170..1921379	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T170265 WP_073660237.1 NZ_CP060392:1915290-1915877 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLVEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T170265 NZ_CP077242:965302-965454 [Escherichia fergusonii]
ATGAAGCAGCATAAGGCGATGATAGTCGCTCTGATCGTCATCTGTGTCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31895.20 Da
• Isoelectric Point: 7.5295

>AT170265 WP_003091607.1 NZ_CP060392:1915944-1916798 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT170265 NZ_CP077242:c965253-965196 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Antitoxin

• AlphaFold DB: A0A1C7C4U7