Detailed information of TA system TA170256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1110445..1111244
• Replicon: chromosome
• Accession: NZ_CP060388
• Organism: Sphingobium sp. KCTC 72723

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: SPBM01_RS05500
• Protein ID: WP_188064359.1
• Coordinates: 1110741..1111244 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: N1MNI2
• Locus tag: SPBM01_RS05495
• Protein ID: WP_006960007.1
• Coordinates: 1110445..1110732 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPBM01_RS05485	1105977..1109720	+	3744	WP_188064357.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
SPBM01_RS05490	1109717..1110250	-	534	WP_188064358.1	hypothetical protein	-
SPBM01_RS05495	1110445..1110732	+	288	WP_006960007.1	DUF1778 domain-containing protein	Antitoxin
SPBM01_RS05500	1110741..1111244	+	504	WP_188064359.1	GNAT family N-acetyltransferase	Toxin
SPBM01_RS05505	1111271..1112185	+	915	WP_188064360.1	HEPN domain-containing protein	-
SPBM01_RS05510	1112567..1114315	+	1749	WP_188064361.1	SIR2 family protein	-
SPBM01_RS05515	1114312..1114938	+	627	WP_188064362.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-92)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18421.94 Da
• Isoelectric Point: 8.9296

>T170256 WP_188064359.1 NZ_CP060388:1110741-1111244 [Sphingobium sp. KCTC 72723]
MAPQFRIEKLRRDHAIDVFDCGREELNRFLSRYAWQAQQGGASQSYVALTGDEVVGFHTLVVGHVDHSDAPDRLTKGMAR
HPVPLMILARLGVSTGYQGKGLGSGLLKDALTRTLQAADIAGIRAIAAHAKDEAARQFYQNFDFIPGLSDPNHMFLLSKD
ARRHLQG

Nucleotide

• Length: 504 bp

>T170256 NZ_CP077238:c2104517-2104335 [Acinetobacter pittii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10466.82 Da
• Isoelectric Point: 5.7066

>AT170256 WP_006960007.1 NZ_CP060388:1110445-1110732 [Sphingobium sp. KCTC 72723]
MASATARTEKLDLRLSPTAKRTLQQAALAAHRSVSEFVLESALARAEETLPDRREFNLDADQWAAFLAALDAPPRHTPRL
AELLTKPSVFEQDPG

Nucleotide

• Length: 288 bp

>AT170256 NZ_CP077238:c2104242-2103838 [Acinetobacter pittii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Antitoxin

• AlphaFold DB: N1MNI2