Detailed information of TA system TA170247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4705209..4705878
• Replicon: chromosome
• Accession: NZ_CP060295
• Organism: Variovorax sp. PAMC26660

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H7F35_RS22140
• Protein ID: WP_187108730.1
• Coordinates: 4705702..4705878 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7F35_RS22135
• Protein ID: WP_187114366.1
• Coordinates: 4705209..4705631 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F35_RS22100	4700546..4701253	+	708	WP_187108724.1	DUF1073 domain-containing protein	-
H7F35_RS22105	4701704..4702015	+	312	WP_187114365.1	tail fiber assembly protein	-
H7F35_RS22110	4702075..4702470	+	396	WP_187108725.1	hypothetical protein	-
H7F35_RS22115	4702467..4702772	+	306	WP_187108726.1	hypothetical protein	-
H7F35_RS22120	4702772..4703254	+	483	WP_187108727.1	glycoside hydrolase family 104 protein	-
H7F35_RS22125	4703322..4703852	+	531	WP_187108728.1	DUF2514 family protein	-
H7F35_RS22130	4703962..4705188	+	1227	WP_187108729.1	class I SAM-dependent methyltransferase	-
H7F35_RS22135	4705209..4705631	-	423	WP_187114366.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H7F35_RS22140	4705702..4705878	-	177	WP_187108730.1	type II toxin-antitoxin system HicA family toxin	Toxin
H7F35_RS22145	4706013..4706357	-	345	WP_187108731.1	hypothetical protein	-
H7F35_RS22150	4706361..4707632	-	1272	WP_187108732.1	site-specific integrase	-
H7F35_RS22155	4708020..4709027	-	1008	WP_187108733.1	hypothetical protein	-
H7F35_RS22160	4709078..4709287	-	210	WP_187108734.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4691146..4707632	16486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-98)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6519.78 Da
• Isoelectric Point: 11.2565

>T170247 WP_187108730.1 NZ_CP060295:c4705878-4705702 [Variovorax sp. PAMC26660]
VKISEFKRWLKTQGVVAVEGKKHSKLSINGKQTVLPRHPSKELKEGTKQAILKQLGLK

Nucleotide

• Length: 177 bp

>T170247 NZ_CP077236:c904677-904264 [Lelliottia amnigena]
GTGGTCCTGTGGCAATCTGATCTGCGCGTATCCTGGCGCTCACAGTGGATGTCTTTGTTGCTCCACGGAATGGTTGCGGC
CTTTGTTTTGCTGATGCCGTGGCCACTCAGCTACACCCCGTTATGGATGCTGTTACTCTCGCTTGTGGTGTTCGACAGCG
TGCGCAGCCAGCGACGTATCAATTCCTGTCAGGGCGAAATCAAGCTGCTGATGGACTCTCGTCTGCGCTGGCAAGGTAAA
GAGTGGGAAATCGTCGGTACGCCCTGGATGCTGAGTTTTGGCATGATGTTTCGCTTGCGTAACGTGCAAGGCGGACGAAA
CCAGCATTTATGGCTTTCGGCGGACAGTATGGATGCTGACGAGTGGCGAGATTTGCGTCGGATGGTTTTGCAGCAACCGA
CGCCCGGTCGATAG

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15557.05 Da
• Isoelectric Point: 5.2961

>AT170247 WP_187114366.1 NZ_CP060295:c4705631-4705209 [Variovorax sp. PAMC26660]
MKFPAKFEPADEGGFVVTFRDVPEAITQGDTLEEAQAMAIDALVTAMDFYFEDGREVPRPSALQKDERLVELPLSVASKV
ELLNIVLKKKARPIDIARSMGIKPQEVTRILDLHHATKIDTMAQAFSALGYRLELKVSKA

Nucleotide

• Length: 423 bp

>AT170247 NZ_CP077236:c904924-904658 [Lelliottia amnigena]
ATGGACATTAGCAATAAGGCTCGTATTCACTGGGCGTGCCGCCGCGGCATGCGCGAACTTGATATTTCCGTCATGCCATT
CTTCGAATATGAATACGACAGCTTAAGCGATGACGATAAGCGTCTGTTTATTCGCCTTCTTGAAAGTGACGATCCTGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA