Detailed information of TA system TA170246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4586981..4587650
• Replicon: chromosome
• Accession: NZ_CP060295
• Organism: Variovorax sp. PAMC26660

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H7F35_RS21490
• Protein ID: WP_187108610.1
• Coordinates: 4587474..4587650 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7F35_RS21485
• Protein ID: WP_187114359.1
• Coordinates: 4586981..4587403 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F35_RS21440	4581995..4582591	+	597	WP_187108601.1	DUF2313 domain-containing protein	-
H7F35_RS21445	4582601..4583908	+	1308	WP_187108602.1	hypothetical protein	-
H7F35_RS21450	4583920..4584090	+	171	WP_187108603.1	hypothetical protein	-
H7F35_RS21455	4584194..4584541	+	348	WP_187108604.1	hypothetical protein	-
H7F35_RS21460	4584534..4584992	+	459	WP_187108605.1	hypothetical protein	-
H7F35_RS21465	4585171..4585527	+	357	WP_187108606.1	hypothetical protein	-
H7F35_RS21470	4585524..4586078	+	555	WP_187108607.1	glycoside hydrolase family 104 protein	-
H7F35_RS21475	4586075..4586680	+	606	WP_187108608.1	hypothetical protein	-
H7F35_RS21480	4586704..4586967	+	264	WP_187108609.1	hypothetical protein	-
H7F35_RS21485	4586981..4587403	-	423	WP_187114359.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H7F35_RS21490	4587474..4587650	-	177	WP_187108610.1	type II toxin-antitoxin system HicA family toxin	Toxin
H7F35_RS21495	4587785..4588129	-	345	WP_187108611.1	hypothetical protein	-
H7F35_RS21500	4588472..4589374	-	903	WP_187108612.1	SDR family NAD(P)-dependent oxidoreductase	-
H7F35_RS21505	4589405..4590436	-	1032	WP_187108613.1	MBL fold metallo-hydrolase	-
H7F35_RS21510	4590546..4591154	+	609	WP_187108614.1	TetR/AcrR family transcriptional regulator	-
H7F35_RS21515	4591209..4591835	-	627	WP_187114360.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-98)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6491.72 Da
• Isoelectric Point: 11.2565

>T170246 WP_187108610.1 NZ_CP060295:c4587650-4587474 [Variovorax sp. PAMC26660]
VKISEFKRWLKTQGVAVAEGKKHSKLSINGKQTVLPRHPSKELKEGTKQAILKQLGLK

Nucleotide

• Length: 177 bp

>T170246 NZ_CP077236:665537-665857 [Lelliottia amnigena]
ATGCATATTTCAACTGTACCGGCCACGGTGCCGGTTTCGCCACGCCTGTCGCCCGTTCAGGTCTGGCAGCAACTGTTAAC
GTACCTACTGGAACACCATTACGGTCTTACGCTCAACGACACACCATTCCATGATGATTCAGCCATCCAGGAACATATCG
AGGCGGGTATCACACTTGCTGATGCGGTGAATTTCCTGGTGGAACGCTATGAACTGGTGCGCATCGACCGTAAAGGATTC
ACCTGGCAAGAGCAGACACCATTCCTGACTACTATCGATTTACTCAGAGCCAGGCGAGCTACCGGATTAGTGCACACATA
A

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15543.02 Da
• Isoelectric Point: 5.2961

>AT170246 WP_187114359.1 NZ_CP060295:c4587403-4586981 [Variovorax sp. PAMC26660]
MKFPAKFEPADEGGFVVTFRDVPEAITQGDTLEEAQAMAVDALVTAMDFYFEDGREVPRPSALQKDERLVELPLSVASKV
ELLNIVLKKKARPIDIARSMGIKPQEVTRILDLHHATKIDTMAQAFSALGYRLELKVSKA

Nucleotide

• Length: 423 bp

>AT170246 NZ_CP077236:665138-665503 [Lelliottia amnigena]
ATGCAATCGACCACCTTATCCGGCTCATCAGCGGAGAACGCATCCTGCCTTCAGTGGGGATTAAAACGTAATATCACGCC
GTGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCGTCTCTACTTTCTGACTGATCGGGCTGGGTTTGATGGCTCATTCA
GTGACGATGAAGCATTGCGTCTGGACCAGGTGTTTCCACTGATGATGAAACAGCTAGAGCGGATGCTCACCACTGGAGAG
CTTGATCCCCGCTGTCAACACTGCGTCACTCTGCATCACAATGGACTCACCTGTGAAGCTGACACTCTCGGCTCTCATGG
TTACGTTTACATCGCTATTTATCCCCAGTCAGCCGTAACCAGGTAA