Detailed information of TA system TA170242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4132135..4132748
• Replicon: chromosome
• Accession: NZ_CP060289
• Organism: Pseudomonas protegens strain H1F10A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7G7XJK7
• Locus tag: GGD92_RS18540
• Protein ID: WP_016962761.1
• Coordinates: 4132566..4132748 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GGD92_RS18535
• Protein ID: WP_177435155.1
• Coordinates: 4132135..4132569 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGD92_RS18520	4127151..4128479	-	1329	WP_179598717.1	LLM class flavin-dependent oxidoreductase	-
GGD92_RS18525	4128622..4130880	-	2259	WP_179598719.1	TonB-dependent receptor	-
GGD92_RS18530	4131086..4132132	+	1047	WP_179598721.1	LacI family DNA-binding transcriptional regulator	-
GGD92_RS18535	4132135..4132569	-	435	WP_177435155.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GGD92_RS18540	4132566..4132748	-	183	WP_016962761.1	type II toxin-antitoxin system HicA family toxin	Toxin
GGD92_RS18545	4133012..4133269	+	258	WP_016962760.1	hypothetical protein	-
GGD92_RS18550	4133279..4135240	-	1962	WP_161799155.1	choline transporter BetT	-
GGD92_RS18555	4135722..4136648	-	927	WP_016962758.1	serine acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (14-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6859.98 Da
• Isoelectric Point: 10.8547

>T170242 WP_016962761.1 NZ_CP060289:c4132748-4132566 [Pseudomonas protegens]
MRSREMIGKIEADGWYLIAVKGSHHQYKHPWKPGRVTIRHPDADLPRGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T170242 NZ_CP077233:c10391-10110 [Acinetobacter pittii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 15956.76 Da
• Isoelectric Point: 4.4600

>AT170242 WP_177435155.1 NZ_CP060289:c4132569-4132135 [Pseudomonas protegens]
MSPLLHEESNDMKFPVVLHKDADSEYGVIVPDVPGCYSAGQTVSQAFENVKQALSLHYEGLVADGEPLPQVHEIDDHLDN
PDYAGGVWGVVEFDVTPYFGKSVRFNATLPEHLLERIDQTVKRDQRYNSRSGFLAAAALRELSA

Nucleotide

• Length: 435 bp

>AT170242 NZ_CP077233:c10694-10392 [Acinetobacter pittii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGTGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A7G7XJK7