Detailed information of TA system TA170234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 662860..664292
• Replicon: chromosome
• Accession: NZ_CP060288
• Organism: Pseudomonas mendocina strain CPS5

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H7683_RS03140
• Protein ID: WP_209450316.1
• Coordinates: 663723..664292 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A550EBN6
• Locus tag: H7683_RS03135
• Protein ID: WP_041979742.1
• Coordinates: 662860..663717 (-)
• Length: 286 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7683_RS03120 (H7683_03120)	659782..660687	-	906	WP_179591827.1	GIDE domain-containing protein	-
H7683_RS03125 (H7683_03125)	660698..661270	-	573	WP_013716744.1	LemA family protein	-
H7683_RS03130 (H7683_03130)	661460..662797	+	1338	WP_013716745.1	imelysin family protein	-
H7683_RS03135 (H7683_03135)	662860..663717	-	858	WP_041979742.1	AraC family transcriptional regulator	Antitoxin
H7683_RS03140 (H7683_03140)	663723..664292	-	570	WP_209450316.1	GNAT family protein	Toxin
H7683_RS03145 (H7683_03145)	664495..667983	+	3489	WP_013716748.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
H7683_RS03150 (H7683_03150)	668039..668833	-	795	WP_231118680.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (17-145)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21469.60 Da
• Isoelectric Point: 7.2799

>T170234 WP_209450316.1 NZ_CP060288:c664292-663723 [Pseudomonas mendocina]
MFKPQLVTLQRGALRLEPLSDADIPALVALAEANREELLYMSGTQRLDWYRSALADLREQRALPFVIRLGDELVGTTRFA
DFMNTLPACEIGWTWLDRNQHGSGLNTTIKYLMLRHAFDNWGMVRVQLKTAASNQRSQRAIEKLGAVREGLLRNHRRLAD
GRLDDTVLYSITDLEWPALREAMEAGFGA

Nucleotide

• Length: 570 bp

>T170234 NZ_CP077212:c38508-38023 [Enterobacter cloacae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 286 a.a.
• Molecular weight: 31735.12 Da
• Isoelectric Point: 9.0437

>AT170234 WP_041979742.1 NZ_CP060288:c663717-662860 [Pseudomonas mendocina]
MQAREQTRFWQASALSGVELLQARYLEQRFAPHVHEGFVFTVIEHGAQRFHHRGSDHLAPQGSMVLINPDEVHTGSKAHE
DGWRYRGFYPGNDQVLGALQELGLAKTGMPSFSFSVLHDPQLHSAFLQLHRLLDGGAEALQQQLAWREAILLLFQRHARL
AEPAAPGREPWAVACAKQMLADRLIEPPSLEELAAAVNLSPFHFARVFRRATGLPPHAWLKQRRLEQARALLKTGCTAVT
VATQLGFADQSHLSRQFKQVYGVSPGEYRSAVARIVAEGAPPGER

Nucleotide

• Length: 858 bp

>AT170234 NZ_CP077212:c38762-38496 [Enterobacter cloacae]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A550EBN6