Detailed information of TA system TA170230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 39266..39791
• Replicon: plasmid pMDR
• Accession: NZ_CP060283
• Organism: Klebsiella pneumoniae strain ent1077

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: H7U30_RS27585
• Protein ID: WP_004197633.1
• Coordinates: 39486..39791 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: H7U30_RS27580
• Protein ID: WP_004197642.1
• Coordinates: 39266..39484 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7U30_RS27535	34372..35367	+	996	WP_019725651.1	hypothetical protein	-
H7U30_RS27540	35440..35763	+	324	WP_019725650.1	hypothetical protein	-
H7U30_RS27545	36197..36391	+	195	WP_019725033.1	hypothetical protein	-
H7U30_RS27550	36435..36665	-	231	WP_019725034.1	hypothetical protein	-
H7U30_RS27555	36679..36882	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
H7U30_RS27560	36916..37284	-	369	WP_015065502.1	hypothetical protein	-
H7U30_RS27565	37328..37822	-	495	WP_009310053.1	hypothetical protein	-
H7U30_RS27570	37853..38425	-	573	WP_015065504.1	hypothetical protein	-
H7U30_RS27575	38422..38670	-	249	WP_019725036.1	hypothetical protein	-
H7U30_RS27580	39266..39484	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H7U30_RS27585	39486..39791	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
H7U30_RS27590	39960..40361	+	402	WP_072199347.1	hypothetical protein	-
H7U30_RS27595	40388..40711	+	324	WP_004197641.1	hypothetical protein	-
H7U30_RS27600	40708..41724	+	1017	WP_004197639.1	hypothetical protein	-
H7U30_RS27605	41922..42716	+	795	WP_004197635.1	site-specific integrase	-
H7U30_RS27610	43172..43351	-	180	WP_006788217.1	hypothetical protein	-
H7U30_RS27615	43471..44097	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / qnrB1 / tet(A)	-	1..111136	111136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T170230 WP_004197633.1 NZ_CP060283:39486-39791 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T170230 NZ_CP077211:c2622365-2622039 [Enterobacter cloacae]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCAGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGGC
GCGTTTGCTGGAACAGCACTACGGTCTGACAATAAACGACCCCCCATTCAGCGATGAAGTTGTTATACAGCAACATATCG
ATGCCGGTATCACCCTGTCAGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
CGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGAGGGCAAGACAGGCAATAGGTCTTTTACGAGGATC
TCATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT170230 WP_004197642.1 NZ_CP060283:39266-39484 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT170230 NZ_CP077211:c2622703-2622386 [Enterobacter cloacae]
ATGACCCACATCATCTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGGCT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGCCGGAAGCTGGATGACACATTCCCAC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5