Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 161421..162091 Replicon plasmid pKpVP
Accession NZ_CP060282
Organism Klebsiella pneumoniae strain ent1077

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag H7U30_RS26915 Protein ID WP_004213072.1
Coordinates 161421..161864 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag H7U30_RS26920 Protein ID WP_004213073.1
Coordinates 161861..162091 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7U30_RS26880 156836..157111 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
H7U30_RS26885 157174..157665 + 492 WP_011251327.1 DM13 domain-containing protein -
H7U30_RS26890 157714..158634 + 921 WP_029884592.1 DUF1471 domain-containing protein -
H7U30_RS26895 158725..159131 + 407 Protein_169 GAF domain-containing protein -
H7U30_RS26900 159645..160275 - 631 Protein_170 response regulator -
H7U30_RS26905 160694..160987 + 294 Protein_171 transposase -
H7U30_RS26910 160991..161407 + 417 Protein_172 restriction endonuclease -
H7U30_RS26915 161421..161864 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
H7U30_RS26920 161861..162091 - 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7U30_RS26925 162699..163832 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
H7U30_RS26930 163848..164141 + 294 WP_004213076.1 hypothetical protein -
H7U30_RS26935 164131..164337 - 207 WP_004213077.1 hypothetical protein -
H7U30_RS26940 164689..164979 + 291 WP_004213078.1 hypothetical protein -
H7U30_RS26945 164969..165868 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA 1..237444 237444


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T170229 WP_004213072.1 NZ_CP060282:c161864-161421 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T170229 NZ_CP077211:2081935-2082348 [Enterobacter cloacae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCGTTATTGCTCCACGGCCTGGTCGCGGC
ATTTGTTTTACTGATGCCGTGGCCGCTTAGCTATACGCCGCTGTGGCTGTTGTTGCTGTCGTTCGTCGTTTTTGACAGCG
TGCGCAGCCAGCGCCGGATCAATGCCCGCCAGGGTGAGATTAAATTACTGATGGACTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAGTTCCGGCATGATGCTACGGTTACGCCAGGTGGGCGGCGGTAAGTG
CCAGCATCTGTGGCTGGCCGCCGACAGTATGGATATTGCGGAGTGGCGGGAACTGCGCCGGATGCTACTGCAGCAGCAAC
CGACGCAGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT170229 WP_004213073.1 NZ_CP060282:c162091-161861 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT170229 NZ_CP077211:2081688-2081954 [Enterobacter cloacae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGACGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGAGGATAAGCGTCTGTTTGTTCGCCTGCTTGAATCTGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q6U619


Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References