Detailed information of TA system TA170227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 42536..43446
• Replicon: plasmid pKpVP
• Accession: NZ_CP060282
• Organism: Klebsiella pneumoniae strain ent1077

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: H7U30_RS26280
• Protein ID: WP_004026354.1
• Coordinates: 42976..43446 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: H7U30_RS26275
• Protein ID: WP_004026357.1
• Coordinates: 42536..42979 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7U30_RS26245	37554..38075	+	522	WP_004213635.1	sigma-70 family RNA polymerase sigma factor	-
H7U30_RS26250	38072..39025	+	954	Protein_40	fec operon regulator FecR	-
H7U30_RS26255	39112..41238	+	2127	WP_029884227.1	TonB-dependent receptor family protein	-
H7U30_RS26260	41313..41468	+	156	WP_004213642.1	hypothetical protein	-
H7U30_RS26265	41613..41834	+	222	WP_004213643.1	DUF2188 domain-containing protein	-
H7U30_RS26270	41932..42435	+	504	Protein_44	DUF4113 domain-containing protein	-
H7U30_RS26275	42536..42979	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
H7U30_RS26280	42976..43446	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
H7U30_RS26285	43901..44107	-	207	WP_004902422.1	hypothetical protein	-
H7U30_RS26290	44703..44963	-	261	WP_004026352.1	hypothetical protein	-
H7U30_RS26295	45534..46715	-	1182	WP_004883380.1	IS481 family transposase	-
H7U30_RS26300	46787..46945	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
H7U30_RS26305	47324..47566	-	243	WP_071826037.1	hypothetical protein	-
H7U30_RS26310	47642..47806	-	165	WP_011154586.1	hypothetical protein	-
H7U30_RS26315	47923..48084	-	162	WP_164507110.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA	1..237444	237444
	-	flank	IS/Tn	-	-	45534..46715	1181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T170227 WP_004026354.1 NZ_CP060282:42976-43446 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T170227 NZ_CP077211:1181394-1181705 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTCCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCGTACCCACGAATTTGAAATCAGATTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT170227 WP_004026357.1 NZ_CP060282:42536-42979 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT170227 NZ_CP077211:1181706-1181996 [Enterobacter cloacae]
ATGGATAAGGCACTGTTTGAGCGATTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGACGTTCGCCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAACGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9