Detailed information of TA system TA170225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5011984..5013665
• Replicon: chromosome
• Accession: NZ_CP060281
• Organism: Klebsiella pneumoniae strain ent1077

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: H7U30_RS24175
• Protein ID: WP_004146663.1
• Coordinates: 5011984..5012901 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: H7U30_RS24180
• Protein ID: WP_004177809.1
• Coordinates: 5012907..5013665 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7U30_RS24155	5007290..5008810	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
H7U30_RS24160	5008807..5009805	+	999	WP_004872882.1	ribose ABC transporter permease	-
H7U30_RS24165	5009836..5010777	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
H7U30_RS24170	5010852..5011859	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
H7U30_RS24175	5011984..5012901	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H7U30_RS24180	5012907..5013665	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
H7U30_RS24185	5013751..5014479	-	729	WP_004177810.1	response regulator transcription factor	-
H7U30_RS24190	5014752..5016899	+	2148	WP_110915727.1	formate dehydrogenase subunit alpha	-
H7U30_RS24195	5017361..5018047	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T170225 WP_004146663.1 NZ_CP060281:c5012901-5011984 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T170225 NZ_CP077211:817454-817741 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCATCGCGTTGGTGGATGATGAGGAAATAGCCGTGTTTATGGCTGATGCATT
AGAAACCGGTGACTCAGCGTATATTGCTAAAGCTTTGGGCGTCATCGCGCGAGCGAAAGGGATGTCGACCATTTCACAGC
AAACTGGCCTGTCACGGGAACAACTGTACCGATCATTTAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTCACAATCAAACCCGCCGGGGATTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT170225 WP_004177809.1 NZ_CP060281:c5013665-5012907 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT170225 NZ_CP077211:817145-817447 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAAGATCGGCGAGCAAGGACCATTATCGC
GTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACATAAAACCCGTGGGGGAAGGTATATGCGAGCTGAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05