Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4722700..4723510 Replicon chromosome
Accession NZ_CP060281
Organism Klebsiella pneumoniae strain ent1077

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag H7U30_RS22825 Protein ID WP_004178461.1
Coordinates 4722700..4723233 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag H7U30_RS22830 Protein ID WP_004222378.1
Coordinates 4723244..4723510 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7U30_RS22820 4721531..4722652 + 1122 WP_002887282.1 cupin domain-containing protein -
H7U30_RS22825 4722700..4723233 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
H7U30_RS22830 4723244..4723510 - 267 WP_004222378.1 DUF1778 domain-containing protein Antitoxin
H7U30_RS22835 4723613..4725046 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
H7U30_RS22840 4725036..4725719 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
H7U30_RS22845 4725891..4727276 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
H7U30_RS22850 4727294..4727638 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T170224 WP_004178461.1 NZ_CP060281:c4723233-4722700 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T170224 NZ_CP077211:817145-817447 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAAGATCGGCGAGCAAGGACCATTATCGC
GTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACATAAAACCCGTGGGGGAAGGTATATGCGAGCTGAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT170224 WP_004222378.1 NZ_CP060281:c4723510-4723244 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT170224 NZ_CP077211:817454-817741 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCATCGCGTTGGTGGATGATGAGGAAATAGCCGTGTTTATGGCTGATGCATT
AGAAACCGGTGACTCAGCGTATATTGCTAAAGCTTTGGGCGTCATCGCGCGAGCGAAAGGGATGTCGACCATTTCACAGC
AAACTGGCCTGTCACGGGAACAACTGTACCGATCATTTAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTCACAATCAAACCCGCCGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References