Detailed information of TA system TA170222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2831451..2833039
• Replicon: chromosome
• Accession: NZ_CP060281
• Organism: Klebsiella pneumoniae strain ent1077

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: H7U30_RS13665
• Protein ID: WP_002904792.1
• Coordinates: 2831451..2832773 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H7U30_RS13670
• Protein ID: WP_004176198.1
• Coordinates: 2832773..2833039 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7U30_RS13650	2826507..2829008	+	2502	WP_029884184.1	fimbrial biogenesis outer membrane usher protein	-
H7U30_RS13655	2828996..2830000	+	1005	WP_029884185.1	type 1 fimbrial protein	-
H7U30_RS13660	2830142..2831434	+	1293	WP_016946381.1	glycoside hydrolase family 10 protein	-
H7U30_RS13665	2831451..2832773	-	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
H7U30_RS13670	2832773..2833039	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H7U30_RS13675	2833426..2833752	-	327	WP_004224757.1	hydroxyisourate hydrolase	-
H7U30_RS13680	2833749..2834249	-	501	WP_004224756.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
H7U30_RS13685	2834227..2835606	-	1380	WP_014599164.1	purine permease	-
H7U30_RS13690	2835952..2837106	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
H7U30_RS13695	2837087..2838010	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T170222 WP_002904792.1 NZ_CP060281:c2832773-2831451 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T170222 NZ_CP077211:c452529-452245 [Enterobacter cloacae]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAA
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATAACCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCCATTGGGAAA
AGAGAGAAATTAACTGTGTATCACGATGCTAACAAACGGCTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT170222 WP_004176198.1 NZ_CP060281:c2833039-2832773 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT170222 NZ_CP077211:c452761-452519 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTGTTTCAGTATGTCGCCGAGACGGGACGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8