Detailed information of TA system TA170205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2504068..2504597
• Replicon: chromosome
• Accession: NZ_CP060278
• Organism: Staphylococcus aureus strain KZN

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H7684_RS12320
• Protein ID: WP_000621175.1
• Coordinates: 2504068..2504430 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: H7684_RS12325
• Protein ID: WP_000948331.1
• Coordinates: 2504427..2504597 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7684_RS12300 (2501046)	2501046..2501816	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
H7684_RS12305 (2501791)	2501791..2502270	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
H7684_RS12310 (2502272)	2502272..2502598	-	327	WP_001052491.1	anti-sigma factor antagonist	-
H7684_RS12315 (2502717)	2502717..2503718	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
H7684_RS12320 (2504068)	2504068..2504430	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H7684_RS12325 (2504427)	2504427..2504597	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
H7684_RS12330 (2504682)	2504682..2505830	-	1149	WP_001281154.1	alanine racemase	-
H7684_RS12335 (2505896)	2505896..2506255	-	360	WP_000581197.1	holo-ACP synthase	-
H7684_RS12340 (2506259)	2506259..2506750	-	492	WP_001205912.1	PH domain-containing protein	-
H7684_RS12345 (2506737)	2506737..2508320	-	1584	WP_001294626.1	PH domain-containing protein	-
H7684_RS12350 (2508313)	2508313..2508792	-	480	WP_001287087.1	hypothetical protein	-
H7684_RS12355 (2509000)	2509000..2509560	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T170205 WP_000621175.1 NZ_CP060278:c2504430-2504068 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T170205 NZ_CP077198:567655-567921 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCGGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT170205 WP_000948331.1 NZ_CP060278:c2504597-2504427 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT170205 NZ_CP077198:567908-568153 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTAGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTGGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGTTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1