Detailed information of TA system TA170200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2306942..2307718
• Replicon: chromosome
• Accession: NZ_CP060278
• Organism: Staphylococcus aureus strain KZN

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: H7684_RS11085
• Protein ID: WP_000031108.1
• Coordinates: 2306942..2307094 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: H7684_RS11090
• Protein ID: WP_001251224.1
• Coordinates: 2307119..2307718 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7684_RS11070 (2303144)	2303144..2303965	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
H7684_RS11075 (2304418)	2304418..2305803	-	1386	WP_000116228.1	class II fumarate hydratase	-
H7684_RS11080 (2305999)	2305999..2306394	-	396	WP_000901023.1	hypothetical protein	-
H7684_RS11085 (2306942)	2306942..2307094	-	153	WP_000031108.1	SAS053 family protein	Toxin
H7684_RS11090 (2307119)	2307119..2307718	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
H7684_RS11095 (2307877)	2307877..2308347	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
H7684_RS11100 (2308352)	2308352..2309479	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
H7684_RS11105 (2309630)	2309630..2310352	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
H7684_RS11110 (2310345)	2310345..2311802	-	1458	WP_031768944.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T170200 WP_000031108.1 NZ_CP060278:c2307094-2306942 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T170200 NZ_CP077198:c545675-545358 [Vibrio cholerae]
ATGCAAGATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTAGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTTGGGAAACACATGGCTTATTACACCAAAGAAGCAGAC
TTTATTCTCATTGTTGCGGTATTAGGGCAATCACAGCTGCCCCAAAAGCATCTCAAACACTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT170200 WP_001251224.1 NZ_CP060278:c2307718-2307119 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT170200 NZ_CP077198:c545936-545694 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAGCGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCCATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTCGATGGTGCCGCTTTTCTAAACACCCTT
TGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4