Detailed information of TA system TA170166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 929410..929939
• Replicon: chromosome
• Accession: NZ_CP060271
• Organism: Staphylococcus xylosus strain DMSX03

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DMSX03_RS04190
• Protein ID: WP_029377956.1
• Coordinates: 929577..929939 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1D4YKY9
• Locus tag: DMSX03_RS04185
• Protein ID: WP_017722121.1
• Coordinates: 929410..929580 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMSX03_RS04160	925187..925666	+	480	WP_099265861.1	PH domain-containing protein	-
DMSX03_RS04165	925659..927167	+	1509	WP_058625515.1	PH domain-containing protein	-
DMSX03_RS04170	927154..927645	+	492	WP_029377959.1	PH domain-containing protein	-
DMSX03_RS04175	927707..928060	+	354	WP_029377958.1	holo-ACP synthase	-
DMSX03_RS04180	928174..929322	+	1149	WP_058625516.1	alanine racemase	-
DMSX03_RS04185	929410..929580	+	171	WP_017722121.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DMSX03_RS04190	929577..929939	+	363	WP_029377956.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DMSX03_RS04195	930288..931289	+	1002	WP_029377955.1	PP2C family protein-serine/threonine phosphatase	-
DMSX03_RS04200	931369..931695	+	327	WP_029377954.1	anti-sigma factor antagonist	-
DMSX03_RS04205	931697..932178	+	482	Protein_829	anti-sigma B factor RsbW	-
DMSX03_RS04210	932153..932923	+	771	WP_038678880.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13539.75 Da
• Isoelectric Point: 10.3173

>T170166 WP_029377956.1 NZ_CP060271:929577-929939 [Staphylococcus xylosus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEKKMKEVNTAIDISLGLHNIRNHKS

Nucleotide

• Length: 363 bp

>T170166 NZ_CP077093:730130-730492 [Pseudomonas vanderleydeniana]
ATGGTACGCAGGCAGGCCCCGCAGCGTGGTGATGTCTATTGGATTGATCCTAATCCGGTGGCTGGTCGAGAAATGATGAA
TCGCCACCGGTTTGTGGTCATCACTCCACGAGAAATCAACGCGTTGGGTGTCAGCATGACCGTTCCCATCACGACTGGCG
GCTCGTTCAGTCGTAATTCCGGTCTGGCGGTTATCGTTACCGGCCATGAGACCAACGGCGTCGCCTTATGCAATCAGGTC
CGCAGCTTCGATATCGAACAGCGCGTACGAGACGGTTCTGCCAAATTCATCGAGCGGTTGGACGATATCACGATGATGGA
TATCGTCTGCCGGGTGGTCAGCGTCATCGACCCCGAAGTGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT170166 WP_017722121.1 NZ_CP060271:929410-929580 [Staphylococcus xylosus]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT170166 NZ_CP077093:729861-730130 [Pseudomonas vanderleydeniana]
ATGTCCGTCACTTCCAAAATTCGTCGTCAAGGTGGCGCTGCCGTCATGACCATTCCTCCTACCCTGCTGAAACTCCTGCA
CCTGGACGTAGGAGCGCAGCTTGAGTTGAAGGTGGTCGATGGCGAGTTGATTGCAAAACCGATGATTCGTCCTGCTCGCA
AACGGTACTCCCTGACTGAGCTGCTTCAGGGGGCCGAGGCGATAGTGGAGTTGAACGCTGAAACCGCGTGGGCTCGTGAA
GGTGACCCGGTTGGAAGGGAGATAGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D4YKY9