Detailed information of TA system TA170156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4919641..4921229
• Replicon: chromosome
• Accession: NZ_CP060263
• Organism: Dickeya zeae strain JZL7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: H7F34_RS22020
• Protein ID: WP_219952226.1
• Coordinates: 4919910..4921229 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H7F34_RS22015
• Protein ID: WP_168364151.1
• Coordinates: 4919641..4919910 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F34_RS21990	4914986..4915597	-	612	WP_219952222.1	NAD(P)-dependent oxidoreductase	-
H7F34_RS21995	4915740..4916126	+	387	WP_168363927.1	helix-turn-helix domain-containing protein	-
H7F34_RS22000	4916263..4917351	-	1089	WP_219952223.1	4-hydroxyphenylpyruvate dioxygenase	-
H7F34_RS22005	4917670..4918443	+	774	WP_219952224.1	MBL fold metallo-hydrolase	-
H7F34_RS22010	4918487..4919464	+	978	WP_219952225.1	helix-turn-helix domain-containing protein	-
H7F34_RS22015	4919641..4919910	+	270	WP_168364151.1	helix-turn-helix domain-containing protein	Antitoxin
H7F34_RS22020	4919910..4921229	+	1320	WP_219952226.1	type II toxin-antitoxin system HipA family toxin	Toxin
H7F34_RS22025	4921262..4922626	-	1365	WP_219952227.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
H7F34_RS22030	4922743..4924374	-	1632	WP_219952228.1	membrane protein insertase YidC	-
H7F34_RS22035	4924377..4924634	-	258	WP_012886807.1	membrane protein insertion efficiency factor YidD	-
H7F34_RS22040	4924598..4924957	-	360	WP_012886808.1	ribonuclease P protein component	-
H7F34_RS22045	4924976..4925116	-	141	WP_000831330.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49397.68 Da
• Isoelectric Point: 7.1335

>T170156 WP_219952226.1 NZ_CP060263:4919910-4921229 [Dickeya zeae]
MAALDVYMNGYRVGILTKTGSGAHHFSYDVNWLGLSGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
ADSTQPFDLLAKVGRDSVGALQLIPASTPTNNIKQIEYKTLSEQQLENILSGYLVDAPLGMIDTEDDFRISIAGAQEKTA
LLYRDNHWCLPFNTTPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAREFGLPVPHCFMLKAGKIKALAVERFDRK
YASDGSWVMRLPQEDFCQVFNVPSALKYENHGGPGISAIMSYLLGAVDPQRDRYSFMKVQVLFWLLAATDGHAKNFSLFI
EPEGRYRLTPFYDILSMHPAMGGRGIDRREAKLAMGLASSKGKKYAIEQIFPRHFFQTAQVVGFARESMESILTEFAQSM
DTIVMKIRNQLPASFPASIRDAILEGMQARAGRLMTGWE

Nucleotide

• Length: 1320 bp

>T170156 NZ_CP077092:c895467-895165 [Pseudomonas iranensis]
ATGATCGCTTTTGAACGATCCCGCATATTTTCCGAATGGCTCGACTCCTTGAAAGACATCAAAGGGAAAGGCCGAATCAT
CTCCAGAATTAAAACAGCCGAAAATGGCAACTTCGGCGATTGCGGGTTCGTCGGTGACACTGTTTATGAAATGCGTGTTC
ATTACGGCCCTGGTTACAGGATGTACTTCACACGCAAAGGCGAAGTGATTTATCTGCTGCTGATCGGGGGCGATAAATCG
ACACAAAAAAGAGATATCAAACGCGCCGTGCAGATGGCTCATAGCATCGGAAATGAGGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10008.27 Da
• Isoelectric Point: 8.5848

>AT170156 WP_168364151.1 NZ_CP060263:4919641-4919910 [Dickeya zeae]
MKVTNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTSRNMDAANNEASV
AESSWKEEW

Nucleotide

• Length: 270 bp

>AT170156 NZ_CP077092:c895161-894871 [Pseudomonas iranensis]
ATGACCGAAGAATTCGCCCGTTTTGACGCCGCCGAGTACCTCAAAACTCCCGAGGACATGGCGGCATATCTAGATGCCTG
TTTTGACGATGACGAAGGCGACGGCGTCCTTATCCGTGCAGCGCTCAATGACATCGCCCGGGCTCAAGGCATGACACAAG
TGGCGCGGGATGCAGGTTTGGGCCGCGAAAGTCTTTATAAAGCCCTGAGTAGCACTGGCAATCCCGAATTCGCAACTATC
ATGAAAGTCATGAAGGCTCTGGGTTTGAGATTGCATGCCGTAACGCTGTGA