Detailed information of TA system TA170153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3136958..3137511
• Replicon: chromosome
• Accession: NZ_CP060263
• Organism: Dickeya zeae strain JZL7

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H7F34_RS14115
• Protein ID: WP_219951506.1
• Coordinates: 3136958..3137272 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: H7F34_RS14120
• Protein ID: WP_216282325.1
• Coordinates: 3137275..3137511 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F34_RS14095	3132027..3132353	+	327	WP_103183781.1	hypothetical protein	-
H7F34_RS14100	3132403..3133119	+	717	WP_219951503.1	hypothetical protein	-
H7F34_RS14105	3133283..3134818	-	1536	WP_219951504.1	ISNCY family transposase	-
H7F34_RS14110	3135243..3136661	+	1419	WP_219951505.1	hypothetical protein	-
H7F34_RS22205	3136731..3136817	+	87	Protein_2778	mobilization protein mobC	-
H7F34_RS14115	3136958..3137272	-	315	WP_219951506.1	CcdB family protein	Toxin
H7F34_RS14120	3137275..3137511	-	237	WP_216282325.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7F34_RS14125	3137832..3138776	-	945	WP_219951507.1	zincin-like metallopeptidase domain-containing protein	-
H7F34_RS14130	3139493..3141802	+	2310	WP_219951508.1	TerB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hcp	3090509..3150374	59865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11521.45 Da
• Isoelectric Point: 7.9859

>T170153 WP_219951506.1 NZ_CP060263:c3137272-3136958 [Dickeya zeae]
MQFTVYGNTGKSAIYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQIKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T170153 NZ_CP077092:c544227-543829 [Pseudomonas iranensis]
ATGATCAAGTACATGCTGGATACAAATATCTGCATCTTCACCATCAAGAACAAACCACAAATTGTCCGGGAAGCCTTCAA
TCGACATTCCGGGCAGCTGTGCATCAGCGCTATCACGCTCATGGAGCTTATTTACGGTGCTGAAAAGTCGGCAGCCCCGG
AGAAAAATCTGGCAATTGTTGAAGGATTTGTCGCGCGACTGGATGTCTTGCCTTTCGATAACGACGCAGCCGCACAGGCC
GGGATGGTTCGATCTGAACTCGCGAAGGCTGGAACGCCGATCGGGCCATACGACCAAATGATCGCTGGCCACGCACGCTC
ACTTGGACTCATTGTAGTGACGAACAACGTACGGGAGTTTCAACGCGTTTCCGGTTTGCGAATCGAGGACTGGGTTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9057.08 Da
• Isoelectric Point: 6.2206

>AT170153 WP_216282325.1 NZ_CP060263:c3137511-3137275 [Dickeya zeae]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTAALDTELRYHEAKKWQEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT170153 NZ_CP077092:c544457-544227 [Pseudomonas iranensis]
ATGGAGCAGACCACAATTTTCATGAGCAACAGAAGTCAGGCAGTCCGACTGCCAAAGGCAGTTGCGATGCCCGGCGACGT
TAAACGAGTGGAAGTCATCGCGATTGGCAGAACACGCGTCATTACGCCTGCTGGAGAAAGCTGGGATAGCTGGTTTGATG
GCGACAGCGTCAGCCCTGATTTCATGGCTAACCGTGAGCAACCCACTGATCAGGAGCGCGAGTCGTTCTGA