Detailed information of TA system TA170138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 468324..469066
• Replicon: chromosome
• Accession: NZ_CP060263
• Organism: Dickeya zeae strain JZL7

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H7F34_RS02120
• Protein ID: WP_027713280.1
• Coordinates: 468324..468803 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A3N0G0N1
• Locus tag: H7F34_RS02125
• Protein ID: WP_019843650.1
• Coordinates: 468794..469066 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F34_RS02090	463545..464426	+	882	WP_219952442.1	integrase domain-containing protein	-
H7F34_RS02095	464429..464629	+	201	WP_026357784.1	AlpA family transcriptional regulator	-
H7F34_RS22050	464626..464952	+	327	Protein_403	ash family protein	-
H7F34_RS22055	464986..465162	+	177	WP_226100054.1	hypothetical protein	-
H7F34_RS22060	465155..465997	+	843	Protein_405	toprim domain-containing protein	-
H7F34_RS22065	466106..467200	+	1095	WP_258235673.1	helicase RepA family protein	-
H7F34_RS22070	467424..467744	-	321	WP_258235601.1	helix-turn-helix transcriptional regulator	-
H7F34_RS02115	467894..468295	-	402	WP_219952445.1	hypothetical protein	-
H7F34_RS02120	468324..468803	-	480	WP_027713280.1	GNAT family N-acetyltransferase	Toxin
H7F34_RS02125	468794..469066	-	273	WP_019843650.1	DUF1778 domain-containing protein	Antitoxin
H7F34_RS02130	469589..470844	+	1256	Protein_411	tyrosine-type recombinase/integrase	-
H7F34_RS02135	470890..471834	+	945	WP_219952446.1	hypothetical protein	-
H7F34_RS02140	471943..472206	-	264	WP_219952448.1	helix-turn-helix transcriptional regulator	-
H7F34_RS02145	472319..472585	+	267	WP_219952450.1	helix-turn-helix transcriptional regulator	-
H7F34_RS02150	472741..473601	+	861	WP_258235602.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	458637..472585	13948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17589.33 Da
• Isoelectric Point: 9.8554

>T170138 WP_027713280.1 NZ_CP060263:c468803-468324 [Dickeya zeae]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRRGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T170138 NZ_CP077090:c1836201-1836016 [Pseudomonas zeae]
ATTGATCACAAAAGCCAAGTAGGTATAGGCCAGCTCGTCGCAGACGGTTGGTCTTTAGTGCGGGTCAGAGGCAGCCACCA
TCACTTCAAACAACCAGTCAAGCCTGGACAGGTCACTGTGCCGCATCCGAAGAAAAATCTGTTGAAGAAAACGGCCATCA
GTATTTTGCAACAAGCGCTGCTCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10388.86 Da
• Isoelectric Point: 4.6702

>AT170138 WP_019843650.1 NZ_CP060263:c469066-468794 [Dickeya zeae]
MRTETKEMPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRVN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT170138 NZ_CP077090:c1835979-1835572 [Pseudomonas zeae]
ATGCTCTACCCGATTGCGATTTCAATAGGTGATGAGGAACACGCCTGGGGCGTGGAGGTGCCGGACATTCCGGGCTGCTT
TTCGGCTGGCGATGATCTGGATGATGCCGTCGCCATGGCTCGCGAAGCCATCGAGGGGCATTTCGAAATTTTGGCGGAGG
ACGGTGCGCCGATCCCGCCTGCGAGCAAACTGGGCGTGAACGTCGCTAATCCGAAGTACGCAGGATGTGCATGGGCCGTG
GTCGATATAGATGTCGTGAAGTACTTGGGTAAAGCCCAGAAACTCAACATCACGTTGCCGGGGCATTTGCTTAACCGCAT
TGACGAATATGTGTTGCACCACCCGGAAGAAAAAAGCCGCTCGGGCTTTCTGGCGTCGGCGGCTCTGAAAGTTTTGCAGC
AGGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3N0G0N1