Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5676554..5677168 Replicon chromosome
Accession NZ_CP060243
Organism Pseudomonas aeruginosa strain A-I-1

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag I5155_RS26950 Protein ID WP_071534354.1
Coordinates 5676986..5677168 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag I5155_RS26945 Protein ID WP_012077229.1
Coordinates 5676554..5676958 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5155_RS26920 5672694..5673404 + 711 WP_012074129.1 hypothetical protein -
I5155_RS26925 5673401..5674321 + 921 WP_012074128.1 hypothetical protein -
I5155_RS26930 5674318..5674857 + 540 WP_012074127.1 hypothetical protein -
I5155_RS26935 5674857..5675555 + 699 WP_033896049.1 hypothetical protein -
I5155_RS26940 5675540..5676511 + 972 WP_012077228.1 hypothetical protein -
I5155_RS26945 5676554..5676958 - 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I5155_RS26950 5676986..5677168 - 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
I5155_RS26955 5677711..5678643 - 933 WP_003120918.1 ZIP family metal transporter -
I5155_RS26960 5678662..5679273 - 612 WP_003098862.1 superoxide dismutase -
I5155_RS26965 5679286..5679735 - 450 WP_003094369.1 hypothetical protein -
I5155_RS26970 5679763..5681139 - 1377 WP_003098863.1 class II fumarate hydratase -
I5155_RS26975 5681132..5681527 - 396 WP_003162782.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5639674..5677168 37494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T170127 WP_071534354.1 NZ_CP060243:c5677168-5676986 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T170127 NZ_CP077086:c6162297-6161932 [Pseudomonas zarinae]
ATGAAATGGGACGTTGAATACACTGACGAATTTGAGCTGTGGTGGGACCGTCTCAGTGAGTCGGAGCAAGACTCGGTACA
AGTCTCGGTGATGCTACTCGGCGATACTGGGCCGTATCTGGGCTTTCCTCACACCAGTGATATCAAAGGTTCGCGTCATG
GCAACCTTCGTGAGTTACGGGTGCAACATGCAGGTAGGCCTTATCGTGTGCTCTATGCGTTTGATCCACGACGCTGCGCC
ATCCTGCTGATCGGGGGAGACAAAACCGGCCAGGACCGTTGGTATCAGCAATATGTGCCTTTGGCCGAACGCCTCTATGA
CGAGCATTTGGAAGTATTGAAGAGAGAGGGCTTTGACCATGGCTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT170127 WP_012077229.1 NZ_CP060243:c5676958-5676554 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT170127 NZ_CP077086:c6161939-6161625 [Pseudomonas zarinae]
ATGGCTAAGAAATTTTCCGATTTGATGGCGCGTCGTTCGCCTGACTCCCAGGCCCGTGCACAGGCTCTGTATCAGCAGTT
GCGTGCGGAAATGCCGCTGCACGAGTTGCGTCAGGCGCAGGAGTTGAGCCAGGAGGCCTTGGCCAAGGCACTGCATATCA
ACCAGGCGGCAGTCTCGAAAATGGAGCGCCGTACGGACATGTACATCAGCACATTGCGCAACTACATCCGGGCCATGGGG
GGGGAATTGGAGATTATCGCGACCTTTCCGGAGGGGCAGGTCAGGATCGAAAATTTCTCCCATCCAACACCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References