Detailed information of TA system TA170121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1174767..1176188
• Replicon: chromosome
• Accession: NZ_CP060243
• Organism: Pseudomonas aeruginosa strain A-I-1

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: I5155_RS05585
• Protein ID: WP_025297643.1
• Coordinates: 1175115..1176188 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: I5155_RS05580
• Protein ID: WP_158025315.1
• Coordinates: 1174767..1175060 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5155_RS05535	1169870..1170082	+	213	WP_023128602.1	hypothetical protein	-
I5155_RS05540	1170173..1170628	-	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
I5155_RS05545	1170793..1171188	+	396	WP_023128603.1	hypothetical protein	-
I5155_RS05550	1171231..1171701	+	471	WP_023128604.1	hypothetical protein	-
I5155_RS05555	1171704..1172087	+	384	WP_025297645.1	hypothetical protein	-
I5155_RS05560	1172169..1172747	+	579	WP_025297644.1	hypothetical protein	-
I5155_RS05565	1173078..1173442	+	365	Protein_1101	DNA binding protein	-
I5155_RS05570	1173666..1173860	-	195	WP_023128607.1	OprD family outer membrane porin	-
I5155_RS05575	1173859..1174554	+	696	WP_196747054.1	transporter substrate-binding domain-containing protein	-
I5155_RS05580	1174767..1175060	-	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
I5155_RS05585	1175115..1176188	-	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
I5155_RS05590	1176903..1177157	+	255	WP_023128610.1	hypothetical protein	-
I5155_RS05595	1177455..1177751	+	297	WP_023128612.1	hypothetical protein	-
I5155_RS05600	1177804..1179387	-	1584	WP_025297642.1	hypothetical protein	-
I5155_RS05605	1179667..1180095	+	429	WP_025297641.1	S24 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1161497..1234044	72547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T170121 WP_025297643.1 NZ_CP060243:c1176188-1175115 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T170121 NZ_CP077085:1921833-1922021 [Pseudomonas shahriarae]
GTGCAAAGCAGGCTATTGATCAAGGAACTGCAAGAGGCGGGCTGGGTGTTGGATCGGGTCATGGGCAGCCATCACATTTT
CACCCATCGCTATAACCCGTCCACGATACCGGTGCCCCATCCCAAGAAGGATCTGCCCTTGGGCACCGTGCGAAGTATCC
GGCAGCGTGCCGGGTTGTTTTCGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT170121 WP_158025315.1 NZ_CP060243:c1175060-1174767 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT170121 NZ_CP077085:1922046-1922450 [Pseudomonas shahriarae]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTACCGCCACCGGTATCCAGATCCCTGATATTCCAGGCGCGGT
GACGGCCGGGGACAGTTTTGAAGAGGCTTACAACGCCGCCGTCGAAGTGGCTCACATCCTGTTGCAGGAGCTGGCTGGCG
AAGGCCGCGCGATTCCGATGCCGACCTCGGTGGCGACGCACCACGGCAATGAGGAGTACGCCGGGATGGGCTGGGGGATG
CTCGAGCTCGATATCTCGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTCACGTTGCCGGGGTATGTGATCCAGCGTAT
TGACCGTTATGTGCGCGAGCACAAGGTCAAAAGTCGCTCGTCATTCCTGGCGGATGCGGCGCTGGAGAAGTTGGTGCGTT
CTTGA