Detailed information of TA system TA170117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4200230..4201488
• Replicon: chromosome
• Accession: NZ_CP060242
• Organism: Pseudomonas aeruginosa strain B-I-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: I5154_RS19555
• Protein ID: WP_003086331.1
• Coordinates: 4200230..4200985 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: I5154_RS19560
• Protein ID: WP_003086329.1
• Coordinates: 4201120..4201488 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5154_RS19545	4195401..4197944	+	2544	WP_003134033.1	acyl-homoserine-lactone acylase QuiP	-
I5154_RS19550	4198099..4199460	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
I5154_RS19555	4200230..4200985	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
I5154_RS19560	4201120..4201488	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
I5154_RS19565	4201561..4202847	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
I5154_RS19570	4202903..4204492	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
I5154_RS19575	4204571..4205047	+	477	WP_003086322.1	hypothetical protein	-
I5154_RS19580	4205050..4205325	-	276	WP_023098713.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T170117 WP_003086331.1 NZ_CP060242:c4200985-4200230 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T170117 NZ_CP077084:4000933-4001283 [Pseudomonas tritici]
ATGGATGCTCTATTTATCGAACTGCCGGCCTTCGAGCGGCATCGCAAGGACTATCTGAGTGACGAGCTTTTCCACGGTTT
TCAACAGGCGTTGATGAAGAACCCGGAAGCGGGAGACGTGATCGAGGGAACAGGCGGGTTACGCAAGGTTCGCTTTGTCG
ACGAACGACGCAACAAAGGCAAGCGCGGTGGCCTGCGGGTCATTTACTACTGGTGGTCGGGCGGCACGCAATTCTGGTTA
TTCACCCTGTACGGCAAACACGAACAGGACGACCTGACGCCACAGCACAAAAAAGCCCTAAAACACAGGCTGGACAGGGA
AATCAAAGCGAGAACACACCATGAAACGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT170117 WP_003086329.1 NZ_CP060242:c4201488-4201120 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT170117 NZ_CP077084:4001273-4001587 [Pseudomonas tritici]
ATGAAACGTGAAATCTTTTCCGAACTGGTCGAAGGCTTCGACGCCCTGGCAAACGAACGCCAAGGCAAAGTCACCCTGCG
GACCCACAAGGTTCATCTCAACAACCTGAAGCCGCTCACCGCAGAGGAAGTGGTCGCCGTGCGCCAACAGCTCAACCTCT
CCCGTGCGGTCTTCGCCATGTACCTGCGCACCAACACGCGTACGCTGGAGAACTGGGAGCAAGGCCGGGCCAAACCCAAC
GCTCAGGCCGCCACCCTGATTCGCCTGGTGGCACGTTTTCCTGAGACCGTGGCGCAACTTGCTGCCTTGGGTTGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7