Detailed information of TA system TA170114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 218283..219504
• Replicon: chromosome
• Accession: NZ_CP060242
• Organism: Pseudomonas aeruginosa strain B-I-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: I5154_RS01150
• Protein ID: WP_003088107.1
• Coordinates: 218283..218861 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I5154_RS01155
• Protein ID: WP_003088112.1
• Coordinates: 218950..219504 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5154_RS01135	213559..214836	+	1278	WP_003088104.1	TolC family protein	-
I5154_RS01140	214826..216997	+	2172	WP_003088105.1	type I secretion system permease/ATPase	-
I5154_RS01145	216987..218174	+	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
I5154_RS01150	218283..218861	-	579	WP_003088107.1	HD domain-containing protein	Toxin
I5154_RS01155	218950..219504	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
I5154_RS01160	219662..221857	-	2196	WP_003088114.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
I5154_RS01165	221862..222323	-	462	WP_003088117.1	(2Fe-2S)-binding protein	-
I5154_RS01170	222654..222977	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
I5154_RS01175	222990..223388	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
I5154_RS01180	223385..223936	-	552	WP_023098678.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T170114 WP_003088107.1 NZ_CP060242:c218861-218283 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T170114 NZ_CP077084:2184468-2184656 [Pseudomonas tritici]
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCAGGCTGGGTGTTGGATCGAGTTACGGGCAGTCATCATATATT
CACCCACCGCTATAACCCGTACACGATCCCCGCTCCCCATCCTAAAAAGGATTTGCCGCTGGGCACTGTCAGAAGCATCA
GAAAACGCGCCGGCCTGTTTGAGTTTTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT170114 WP_003088112.1 NZ_CP060242:c219504-218950 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT170114 NZ_CP077084:2184668-2185072 [Pseudomonas tritici]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATCCCAGGCGCGGT
CACGGCCGGGGACAGTTTTGAAGAGGCCTACAAGGCCGCAGTGGAAGTCGCGCACATCAGGCTGCAAGAGATTGCCGCAG
AGGGTGGGCCGATTCCGATGCCAACGTCGGTCGCCAATCATCACGTGCACGAAGACTACGCCGGCATGGGTTGGGGGATG
TTGGAATTGGACATCTCGCCCTACCTGGGCAAGACCGAGAAGGTCAATGTGACGTTGCCTGGTTATGTCATCCAGCGCAT
CGACCGTTATGTGCGTGAGCATAAGATCAAGAGCCGTTCTTCCTTCCTGGCGGATGCTGCGCTTGAGAAGCTGGTGCGGT
CCTGA

Structures

Toxin

• AlphaFold DB: V6AG16