Detailed information of TA system TA170107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4691886..4692472
• Replicon: chromosome
• Accession: NZ_CP060240
• Organism: Pseudomonas aeruginosa strain G-I-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: I5102_RS21970
• Protein ID: WP_003120987.1
• Coordinates: 4692173..4692472 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: I5102_RS21965
• Protein ID: WP_003448662.1
• Coordinates: 4691886..4692176 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5102_RS21945	4687038..4687247	+	210	WP_003105733.1	cold-shock protein	-
I5102_RS21950	4687468..4689357	+	1890	WP_031641300.1	hypothetical protein	-
I5102_RS21955	4689354..4691330	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
I5102_RS21960	4691471..4691815	+	345	WP_016851612.1	hypothetical protein	-
I5102_RS21965	4691886..4692176	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
I5102_RS21970	4692173..4692472	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5102_RS21975	4692674..4693798	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
I5102_RS21980	4693798..4695507	+	1710	WP_034037331.1	PilN family type IVB pilus formation outer membrane protein	-
I5102_RS21985	4695511..4696836	+	1326	WP_031806139.1	type 4b pilus protein PilO2	-
I5102_RS21990	4696826..4697359	+	534	WP_004352829.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4664629..4765716	101087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T170107 WP_003120987.1 NZ_CP060240:c4692472-4692173 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T170107 NZ_CP077083:2452502-2452756 [Pseudomonas salmasensis]
ATGGAAACTATCAACTACACCAACGCCCGAGCCCATCTGTCCGAAACGATGGACCGGGTCAACGATGATCACGTCCCACT
GTTACTGACTCGACAAAAAGGCGAGCCGGTGGTGATGATCTCGTTATCAGAGTTCAATGCTTTGGAAGAGACGGCTTACT
TGCTGCGCTCGCCTAAAAATGCCGAACGTCTCATTAACTCAGTCAACAGTTTACGAGCAGGCAAAGCCAAGCCAAGGCGG
TTGATTGAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT170107 WP_003448662.1 NZ_CP060240:c4692176-4691886 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT170107 NZ_CP077083:2452753-2453007 [Pseudomonas salmasensis]
ATGAAAATACTTTTTACCCCTGAAGGCTGGGACGACTACCTCTGGTTTCAACAGAACGACAAAGCCGGGCTCAAGCGCAT
CAACCTCTTGATCAAGGCCATACAGAGAGACCCGTTCGACGGCGTGGGAAAGCCTGAACCTCTCAAACATAATCTGAGTG
GTTTCTGGTCTCGGCGAATCACCGCGGAGCATCGTTTGGTTTATGGCATCGAAGATGATGAAGTACAAATATTGATGTGC
CGCTACCACTACTGA

Structures

Toxin

• AlphaFold DB: G8CP73