Detailed information of TA system TA170103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1935514..1937022
• Replicon: chromosome
• Accession: NZ_CP060240
• Organism: Pseudomonas aeruginosa strain G-I-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: I5102_RS08990
• Protein ID: WP_003119682.1
• Coordinates: 1935514..1936101 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1C7C4U7
• Locus tag: I5102_RS08995
• Protein ID: WP_003091607.1
• Coordinates: 1936168..1937022 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5102_RS08985	1931769..1935248	-	3480	WP_003117605.1	hybrid sensor histidine kinase/response regulator	-
I5102_RS08990	1935514..1936101	+	588	WP_003119682.1	GNAT family N-acetyltransferase	Toxin
I5102_RS08995	1936168..1937022	+	855	WP_003091607.1	AraC family transcriptional regulator	Antitoxin
I5102_RS09000	1937119..1939284	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
I5102_RS09005	1939459..1941333	+	1875	WP_003117607.1	MFS transporter	-
I5102_RS09010	1941394..1941603	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T170103 WP_003119682.1 NZ_CP060240:1935514-1936101 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T170103 NZ_CP077082:c5597178-5596603 [Pseudomonas sp. OE 28.3]
ATGAGGTGTTCGCCGTTTACTGCGGTATACGATGCGTGTGTTCTCTACCCGGCGGCACTCCGGGATTTGCTGATGTGGCT
TGGTTTGTCGGGACTGTATCGAGCTCGATGGACGAGCCAGATCCATGAGGAGTGGAAGCGCAACGTTCTCAAAAACCGAC
CGGATCTTACGGCTGAGCAAGTCAATTGCACGTCGGCGCTGATGGATAAGGCGATACCCGATGCATTGGTGACGGGGTAC
GAGGATCTGTGTTCGAGTTTGGATTTGCCTGATCCTGATGACCGTCATGTGCTGGCGGCGGCGATTCGAAGCAAGGCTGA
AGTTATCGTGACCTTCAATTTGAAAGATTTCCCGGCGCATATCCTGGCTGCCTATGACATTGAGCCTATGCATCCGGATG
ACTTTATTTCGGATCTGTGGGATTTGGATCAGGCGGCGGTGTTGGGGGCGGCGCAGAAGCAAAGGCGGACGTTGAAGCGG
CCGCCGATGGAGGCTGGGAGTTATCTTGAGATGTTGATGCGCCAGGGGTTGGTGCAGACGAGTAAGGTGCTTGGGCCATA
TGGGGTGATGTTGTAG

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31895.20 Da
• Isoelectric Point: 7.5295

>AT170103 WP_003091607.1 NZ_CP060240:1936168-1937022 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT170103 NZ_CP077082:c5597645-5597175 [Pseudomonas sp. OE 28.3]
ATGATCACCAATCAACTCCCCAAAACGATCCTGCCGCTGGAAAAGGAAGTCGAAGCCGCCGTGCAAGGCCAGCGAGAACT
GGCATCTTTGCTTTCGACCAAATTTGAAACCCAGCGCATCGACATCTTCGACAAAGAAGACAAACCCCACACACTTGTAC
TCCCGACCTCAGCCCTGCGCCTGTTGGTCGACATCCTGGGCGAATTGGCTATCGGTAACGCCGTCAAGGTCGTGCCGGTG
CATGCCGAATTGACCTCTCAGGAGGCCGCCGACCTGCTCAACGTTTCGCGCCCGCACCTGGTCAAGATGCTGGAGGAGGG
CGCCATTCCTTTTACAAAAACGGGCCGGCATCGGCGAATCCGGTTTTCGGATCTGATGGCGTTCAAGCAGCGCCGTGATG
AAGAAAGCCAAGAGGCAATGGAAGCGCTGGCGCAACAAGCTCAGGCGTTGGGAATGGGGTATGACGGATGA

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ

Antitoxin

• AlphaFold DB: A0A1C7C4U7