Detailed information of TA system TA170094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 65916..66718
• Replicon: plasmid pMDW2
• Accession: NZ_CP060199
• Organism: Aminobacter sp. MDW-2

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: H5P29_RS31445
• Protein ID: WP_185910464.1
• Coordinates: 66191..66718 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: H5P29_RS31440
• Protein ID: WP_154387124.1
• Coordinates: 65916..66194 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5P29_RS31420	61235..62242	-	1008	WP_154387121.1	plasmid partitioning protein RepB	-
H5P29_RS31425	62242..63435	-	1194	WP_185910470.1	plasmid partitioning protein RepA	-
H5P29_RS31430	64200..64523	-	324	Protein_69	PIN domain-containing protein	-
H5P29_RS31435	64701..65816	-	1116	WP_154387123.1	site-specific integrase	-
H5P29_RS31440	65916..66194	+	279	WP_154387124.1	DUF1778 domain-containing protein	Antitoxin
H5P29_RS31445	66191..66718	+	528	WP_185910464.1	GNAT family N-acetyltransferase	Toxin
H5P29_RS31450	67001..67963	+	963	WP_154387125.1	DUF1403 family protein	-
H5P29_RS31455	67969..68679	+	711	WP_154387126.1	SMC-Scp complex subunit ScpB	-
H5P29_RS31460	68738..71389	+	2652	WP_154387127.1	PD-(D/E)XK nuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157689	157689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18809.71 Da
• Isoelectric Point: 8.2950

>T170094 WP_185910464.1 NZ_CP060199:66191-66718 [Aminobacter sp. MDW-2]
VNVPPWHEAAIARSHDRKAFDCGDVEMNGFLQRFARQGHEQNAVKTYCAVPDDAPTRVLGFYSLAPASVEHDAVPAAMTK
GMARHAVPGFLLARLAVDQSLAGRGLGGQLLLAAALRCIRVTVEVGGVLMIIDAKNERAANWYARYGAEPLKDRPLTLVV
PLATFAKALEATGHL

Nucleotide

• Length: 528 bp

>T170094 NZ_CP077082:c1809938-1809654 [Pseudomonas sp. OE 28.3]
ATGCAGGTTGATTGGCTGAGGGCTGCCCTCAGGAATCTTGACGATGAAGCGGCCTACATTGCCGAAGAAAACCCGAAAGC
TGCCCATGAGTTCGTGCAGGCGATCTTGACCGGGCTTGAACATGTCGCTGAATTTCCGGCAATGGGCAAAGAAGGCAGAG
TGCCCGGCACGCGCGAATGGGTAGTGCCCCGATGGCCCTATATCGTTCCCTACCGTATTCGAGATGGCCGGTTACAGGTG
ATGCGAATTTTTCATACACGCCGCCAGCCACCCGCTTCCTGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10145.77 Da
• Isoelectric Point: 10.8838

>AT170094 WP_154387124.1 NZ_CP060199:65916-66194 [Aminobacter sp. MDW-2]
MPRTALDDTDRMNIRVKPEVKARLLRAAALRHTDLTSFVTQSALREAETVIAEADAIKVSERDFGRILTLLDNPPKPNAR
LRAAAAALPKTL

Nucleotide

• Length: 279 bp

>AT170094 NZ_CP077082:c1810167-1809928 [Pseudomonas sp. OE 28.3]
ATGTCTGTTATGTCTCTACGCATACCGGAAGACGTCGCCGACACGCTTGCCAGTCTCGCCAAGGCGACAGGGCGCAGCAA
ATCCTTTCTAGCCGTAGATGCATTACGCGAATACCTGACGCGTGAGGCCTGGCAAATAGAGGAAATTCAAAAAGCATTGA
AAGAAGCTGATGCGGGCGACTTTGCGACAGCGGCCGAAGTAAAAGCAGTCGCCGATAAATGGCGTAACAATGCAGGTTGA