Detailed information of TA system TA170092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5416346..5418036
• Replicon: chromosome
• Accession: NZ_CP060197
• Organism: Aminobacter sp. MDW-2

Toxin (Protein)

• Gene name: HipA2
• Uniprot ID: -
• Locus tag: H5P29_RS26730
• Protein ID: WP_154385356.1
• Coordinates: 5416346..5417695 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: H5P29_RS26735
• Protein ID: WP_154385396.1
• Coordinates: 5417701..5418036 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5P29_RS26700	5411504..5412842	+	1339	Protein_5286	P-type conjugative transfer protein TrbL	-
H5P29_RS26705	5412839..5413528	+	690	WP_154385358.1	conjugal transfer protein TrbF	-
H5P29_RS26710	5413525..5414505	+	981	WP_067965104.1	P-type conjugative transfer protein TrbG	-
H5P29_RS26715	5414502..5415650	+	1149	WP_154385357.1	TrbI/VirB10 family protein	-
H5P29_RS26720	5415647..5415877	+	231	WP_067965108.1	DUF2274 domain-containing protein	-
H5P29_RS26725	5416038..5416295	+	258	WP_083948686.1	AlpA family phage regulatory protein	-
H5P29_RS26730	5416346..5417695	-	1350	WP_154385356.1	HipA domain-containing protein	Toxin
H5P29_RS26735	5417701..5418036	-	336	WP_154385396.1	helix-turn-helix transcriptional regulator	Antitoxin
H5P29_RS26740	5419061..5420317	-	1257	WP_067965111.1	site-specific integrase	-
H5P29_RS26745	5420611..5422173	-	1563	WP_067965113.1	glutamine-hydrolyzing GMP synthase	-
H5P29_RS26750	5422271..5422729	-	459	WP_067965115.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-106)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49012.78 Da
• Isoelectric Point: 5.5530

>T170092 WP_154385356.1 NZ_CP060197:c5417695-5416346 [Aminobacter sp. MDW-2]
VDLLALDVRLDGFDDPVGVFVRDERGAVAFGYNRRYLANPDAAPLSLSLPLVGGPYPDVAARAFFDNLLQERDGALTEVM
EREGLARDDIAGLLFHLGKDCAGALSVLPMGSPPAKVPGDYERDYVPIPPDRLAAIVEALHNRTRLPTGMEDPSPLAGVQ
SKIALTVLPDGRFAEPNPGSGAPTTHILKVPDQGHKRDARYEAEAMDLSRKLGMETAEATALPVGGIEALLVTRFDRGRD
RLGRVVRLHQEDFAQALGLPASMKYERRGREGRRFDVSGIRKILEQTVDPARAKEAFIQATIFDLLVGNADGHAKNFALL
YEPGGRVRLAPRYDILPTRLDDSLTDELPYSIGEAKTLDEVTPSALDAFFQALGIENAAGRRRLAQRYARDIAFALASHL
KALEKNEKRFADLIASNIRALLGALEIEIPDAARERDAFISKAGGWHMS

Nucleotide

• Length: 1350 bp

>T170092 NZ_CP077081:c6038092-6037814 [Pseudomonas atacamensis]
ATGATCGTTAGCTTCAGATGTCCTGAAACCGAATACCTGTTCCGAAGTGGTAAAACCCGTTTCTGGTCAGCCATATTGAG
TGTCGCGGAACGTAAGCTGACGATGCTTGATGCGGCAGCTGCGTTGGCTGATCTCCGATCTCCTCCCGGAAATCGGCTCG
AGTCTCTGGGAGGCGATCGGAAGGGGCAATGCAGTATTCGTATCAACGCCCAATGGCGAATTTGCTTCGTCTGGGGCCTG
AATGGGCCCGAGGATGTTGAAATCGTCGATTATCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12209.83 Da
• Isoelectric Point: 10.7725

>AT170092 WP_154385396.1 NZ_CP060197:c5418036-5417701 [Aminobacter sp. MDW-2]
MPQGVRVSLPHSVQDSYKQQNRATVDDNAIASRAIRDTEAEGLEVVSPSDLGLLVRRARETRHLSQQSFADLAGVGRRFL
SELENGKPTLELGKVLKVARTAGISLFARQR

Nucleotide

• Length: 336 bp

>AT170092 NZ_CP077081:c6037806-6037501 [Pseudomonas atacamensis]
ATGACCAAGAATGGTATGCGTCCGGTCCATCCCGGCGAGATCCTCAAAGAGGAGTATCTGGAGCCTTTGAGCCTTACAGC
CGCTGCGTTGGCGAGAGCATTGAGCGTGTCTACTCCGACGGTGAACGATATCGTTCTACAGCGCCGGGGCATCAGCGCTG
ACATGGCTTTAAGACTTTCAATCTGTCTGGACACCACTCCTGAGTTTTGGCTGAACCTCCAATCGACCTACGATCTGCGA
AAAGCCGAAATTGAAAGAGGAACTGCAATCCGTGATCAGGTCGAGCGGCTGGCACATTGCGCTTGA