Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3622829..3623465 Replicon chromosome
Accession NZ_CP060193
Organism Bacillus sp. PAMC26543

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag H7F27_RS18345 Protein ID WP_003156187.1
Coordinates 3622829..3623179 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag H7F27_RS18350 Protein ID WP_003225183.1
Coordinates 3623184..3623465 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7F27_RS18305 3617874..3618473 - 600 WP_024120304.1 phosphoserine phosphatase RsbX -
H7F27_RS18310 3618473..3619261 - 789 WP_024120303.1 RNA polymerase sigma factor SigB -
H7F27_RS18315 3619227..3619709 - 483 WP_024120302.1 anti-sigma B factor RsbW -
H7F27_RS18320 3619706..3620035 - 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
H7F27_RS18325 3620096..3621103 - 1008 WP_024120300.1 phosphoserine phosphatase RsbU -
H7F27_RS18330 3621115..3621516 - 402 WP_024120299.1 serine/threonine-protein kinase RsbT -
H7F27_RS18335 3621520..3621885 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
H7F27_RS18340 3621890..3622714 - 825 WP_081638235.1 RsbT co-antagonist protein RsbRA -
H7F27_RS18345 3622829..3623179 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H7F27_RS18350 3623184..3623465 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
H7F27_RS18355 3623583..3624752 - 1170 WP_024120297.1 alanine racemase -
H7F27_RS18360 3624869..3625885 - 1017 WP_082687822.1 outer membrane lipoprotein carrier protein LolA -
H7F27_RS18365 3626051..3626416 - 366 WP_024120295.1 holo-ACP synthase -
H7F27_RS18370 3626511..3627110 + 600 WP_024120294.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T170091 WP_003156187.1 NZ_CP060193:c3623179-3622829 [Bacillus sp. PAMC26543]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T170091 NZ_CP077081:5515095-5515739 [Pseudomonas atacamensis]
ATGAACTACACCAGCCTGAATCCAGAGAAAGCTCTGATTTGGCGTATCGTCCATCGCGATAACCTGCCCTGGATTCTGGA
CAACGGTTTGCATTGCCCAACCTCGGAAGCACAGGCGCCTGAGTACGTGAATATCGGCAACGTCGACTTGATCGACAAGC
GCCGTTCCCGTCAGGTGCCTATTGCACCCGAGGGCGTTCTGGCCGATTACGTGCCCTTCTATTTCACACCCTTCTCGGTG
ATGATGCAGAACATTCACTCCGGCTGGAGTGTTCAGCAACGCAGCAACGACGAGATCGTGATTCTGGTCTCAAGCCTGTA
TCGCGTTAAAGAGCTTGGCCTGCCGTTTGTCTTCACCAACGCACATGCCTATCCGAGTTGGACAAACTATTACAGTGATC
TGGCGAATCTCGACCAGATCGATTGGTCTATCCTTCAACGACGCGACTTCAAGCGCGACCCTGATGACCCTCGCAAAATG
GAGCGTTATCAGGCTGAGGCGCTGATCCACCATCACTTACCGATTACGGGACTACTGGGCATCATGTGCCACACCGATGC
AATGAAAGTACGCATAGAAGCAGACGTCGCAGCCAGAGGCCTGACGTTGCCTGTCCATGCGCGTCCCAAATGGTATTTCC
AATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT170091 WP_003225183.1 NZ_CP060193:c3623465-3623184 [Bacillus sp. PAMC26543]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT170091 NZ_CP077081:5515736-5516821 [Pseudomonas atacamensis]
ATGATCAGATTCACCCAAGGCAACCTGCTGGAAGCCAAAACAGAAGCCCTCGTCAACACGGTGAATACCGTAGGCGTGAT
GGGCAAGGGCATCGCATTGATGTTCAAAGAGCGCTTTGCGCAGAATTACCGCGTGTATGCAGCGGCGTGTAAGGCCGGGG
AAGTAGAAACCGGCAAAATGCATGTGACTGCCACCAACGAACTCGACGGCCCTCGCTGGATCGTGAATTTCCCCACCAAG
CGACACTGGCGGTCGCCGTCGCAGATGGCATGGATCACCGAAGGTTTGCATGACCTGCGGCGGTTCCTGCTTGAAAACGA
TGTGAAATCCGTTGCGGTTCCACCGTTAGGTGCTGGTAACGGCGGTTTGAAGTGGCCTGACGTCCGTGAACAGATTGTCG
AAGTGTTGGGAGATCTCGATGCTGATGTCTTGGTGTTCGAGCCGTCCAATCAGTACCTGAATGTCGCCAAACGCGCGGGC
GTAGAAAAGCTCACGCCAGCCCGGGCGCTTATCGCCGAACTGGTTCGTCGCTACTGGGTTTTAGGCATGGAGTGCAGTCT
GCTGGAGATTCAGAAGCTCGCCTGGTTTCTCGAGCGCGCTATCGAGCAGCTGCCAGATACCGAGAACCCTCTGAACCTGA
AGTTCGTCGCACATAAATATGGCCCATACGCCAACCGGCTTGAACACCTGCTTGATAACCTCGATGGCAGCTATCTGCAC
TGTGACAAGCGCATCAGCGACGCAGGCATTAATGATGTGATCTGGTTTGATCAAGATCGCAAAGCGTTTTTACAAACGTA
TCTCAAGACTGAGGCCAAGGCATATTCGCAAGCGTTGGAACGTACGGCGCAACTGATTGACGGTTTTGAATCGCCATTCG
GTATGGAGTTATTGGCCACGGTCGATTGGTTGTTGAGCCAGGAAGGGGTATCTGCGACTGTTCCAGCGCTACGCGAAGCA
TTGAAGCATTGGGAAGGTGGCGCCGGTGCTGCGGCTCGCAAGAGCAAACTGTTCGATGATCAGGCCCTTGATATTGCGCT
CAAGCGCCTGACATCCAGTACTTTCCGGCCTGGATTAGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References