Detailed information of TA system TA170090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2793631..2794547
• Replicon: chromosome
• Accession: NZ_CP060193
• Organism: Bacillus sp. PAMC26543

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A7G7U9Y2
• Locus tag: H7F27_RS13990
• Protein ID: WP_024121091.1
• Coordinates: 2793631..2794377 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A7G7U9Y3
• Locus tag: H7F27_RS13995
• Protein ID: WP_024121090.1
• Coordinates: 2794377..2794547 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F27_RS13970	2788955..2789905	-	951	WP_024121094.1	ring-cleaving dioxygenase	-
H7F27_RS13975	2790300..2791616	+	1317	WP_185848044.1	serine/threonine exchanger	-
H7F27_RS13980	2791892..2792509	+	618	WP_010333926.1	DUF47 domain-containing protein	-
H7F27_RS13985	2792522..2793523	+	1002	WP_024121092.1	inorganic phosphate transporter family protein	-
H7F27_RS13990	2793631..2794377	+	747	WP_024121091.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
H7F27_RS13995	2794377..2794547	+	171	WP_024121090.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H7F27_RS14000	2794631..2794768	+	138	WP_125825426.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
H7F27_RS14005	2794804..2795693	-	890	Protein_2751	N-acetylmuramoyl-L-alanine amidase	-
H7F27_RS14010	2795706..2795969	-	264	WP_024121086.1	phage holin	-
H7F27_RS14015	2795981..2796250	-	270	WP_024121085.1	hemolysin XhlA family protein	-
H7F27_RS14020	2796306..2797145	-	840	WP_185848045.1	phage portal protein	-
H7F27_RS14025	2797189..2797353	-	165	WP_185848046.1	XkdX family protein	-
H7F27_RS14030	2797350..2797679	-	330	WP_024121082.1	XkdW family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2783727..2827843	44116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29076.48 Da
• Isoelectric Point: 4.4986

>T170090 WP_024121091.1 NZ_CP060193:2793631-2794377 [Bacillus sp. PAMC26543]
MLLFFQIMVWCTMAALGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMIYWTYDPSSLFTNWERYVIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLDRLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CHYSTQSDKDRLTEHMEDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQSYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T170090 NZ_CP077081:5413317-5413595 [Pseudomonas atacamensis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACAGGTACGACTCGTGGAATTCGTGCCGACCATGC
AAAACGGCTGTCGCGCATGTTGCAGTTCATGGATCGAGCGACACTTCCCGGCGATCTGGACTTGCCGGGCTGGCGCCTGC
ATCCGTTGAAAGGCGAGCTGAGTGAATACTGGTCGCTCAGCGTTTCAGGCAACTGGCGAGTGATCTTTCGTTTTGTCGGT
TCGGATATCGAATTGGTCGATTATCTGGACTACCACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6578.62 Da
• Isoelectric Point: 10.9236

>AT170090 WP_024121090.1 NZ_CP060193:2794377-2794547 [Bacillus sp. PAMC26543]
MENAFQNGCHPRAKKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT170090 NZ_CP077081:5413610-5413897 [Pseudomonas atacamensis]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACCCTGCTGCTGGATGTCTTGCCCGAGCTTGGTATCAGCGTGAGCGA
ATTGGCCCGGCACCTTGGCTTTGCACGCCCGCATCTTTCACGCGTACTGCACGGACATGCGCCAATCAGCCCGGATCTGG
CAGTACGACTTGAGCGTGCAGGGATCGGCAAGGCGCGCGTTTGGCTAGGCGTCCAAACCGACTACGATCTTTGGCAAGCG
GAGCACCGTGAGCAACCCATGATAGAACCCCTCGCAGCCCATGGCTGA

Structures

Toxin

• AlphaFold DB: A0A7G7U9Y2

Antitoxin

• AlphaFold DB: A0A7G7U9Y3