Detailed information of TA system TA170089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2607313..2608232
• Replicon: chromosome
• Accession: NZ_CP060192
• Organism: Bacillus sp. PAMC28748

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: H7F26_RS13340
• Protein ID: WP_003180879.1
• Coordinates: 2607486..2608232 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: H7F26_RS13335
• Protein ID: WP_003180877.1
• Coordinates: 2607313..2607486 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F26_RS13315	2602738..2603835	+	1098	WP_003180867.1	mannonate dehydratase	-
H7F26_RS13320	2603811..2604659	+	849	WP_009328728.1	SDR family oxidoreductase	-
H7F26_RS13325	2604686..2605699	+	1014	WP_003180872.1	zinc-binding alcohol dehydrogenase family protein	-
H7F26_RS13330	2605752..2607023	+	1272	WP_011197811.1	MFS transporter	-
H7F26_RS13335	2607313..2607486	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H7F26_RS13340	2607486..2608232	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
H7F26_RS13345	2608343..2609341	-	999	WP_009328723.1	inorganic phosphate transporter	-
H7F26_RS13350	2609354..2609971	-	618	WP_003180884.1	DUF47 domain-containing protein	-
H7F26_RS13355	2610303..2612060	+	1758	WP_003180886.1	gamma-glutamyltransferase	-
H7F26_RS13360	2612189..2612833	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T170089 WP_003180879.1 NZ_CP060192:c2608232-2607486 [Bacillus sp. PAMC28748]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T170089 NZ_CP077081:c1751104-1750925 [Pseudomonas atacamensis]
GTGAACAGCCGTTTTTTAATCGGACAACTCGTCGCGGACGGTTGGTTTCTGGTACGTATTCGGGGCAGTCATCATCACTT
CAAACATCCAACCAAACCAGGATTGGTAACAGTGCCGCATCAAAAAAAGGATCTGCTCAAAAAAACGGCCATCAGTATTT
TGCAACAGGCCATGCTCTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT170089 WP_003180877.1 NZ_CP060192:c2607486-2607313 [Bacillus sp. PAMC28748]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT170089 NZ_CP077081:c1750889-1750482 [Pseudomonas atacamensis]
ATGCTTTACCCGATTGCGATTTCAATAGGCGATGAGGAACACGCTTGGGGAGTTGAAGTGCCGGACATTCCTGGCTGCTT
TTCTGCCGGCGACGACCTTGATGACGCTGTCGCCATGGCGCGCGAGGCGATCGAAGGTCATTTCGAAATTCTGGCATACG
ACGGTGCGCCGATTCCATCGGCCAGCAAATTGAGCCTGCATGTCGCCAATCCGCAGTACGCCGGATGTGCGTGGGCGGTA
GTTGATATCGACGTGGTCAAATACCTGGGCAAAGCACAGAAGCTCAACATCACTCTGCCGGGGCACTTGCTCAATCGCAT
TGATGAGTACGTGTTACATCATCCTGAGGAGAAAAGCCGATCGGGGTTTCTGGCTTCGGCGGCGTTGAAGGTCTTGCAGC
AGGGTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37