Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 222991..223627 Replicon chromosome
Accession NZ_CP060191
Organism Bacillus sp. PAMC28571

Toxin (Protein)


Gene name mazF Uniprot ID A0A0P7G713
Locus tag H7F25_RS00880 Protein ID WP_024425388.1
Coordinates 223277..223627 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag H7F25_RS00875 Protein ID WP_003214273.1
Coordinates 222991..223272 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7F25_RS00855 219137..219742 - 606 WP_103132679.1 rhomboid family intramembrane serine protease -
H7F25_RS00860 219837..220202 + 366 WP_041089202.1 holo-ACP synthase -
H7F25_RS00865 220363..221379 + 1017 WP_087975235.1 outer membrane lipoprotein carrier protein LolA -
H7F25_RS00870 221521..222696 + 1176 WP_103132680.1 alanine racemase -
H7F25_RS00875 222991..223272 + 282 WP_003214273.1 hypothetical protein Antitoxin
H7F25_RS00880 223277..223627 + 351 WP_024425388.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H7F25_RS00885 223744..224574 + 831 WP_044334717.1 RsbT co-antagonist protein RsbRA -
H7F25_RS00890 224579..224947 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
H7F25_RS00895 224950..225351 + 402 WP_073203956.1 anti-sigma regulatory factor -
H7F25_RS00900 225362..226369 + 1008 WP_024425391.1 PP2C family protein-serine/threonine phosphatase -
H7F25_RS00905 226429..226758 + 330 WP_024425392.1 anti-sigma factor antagonist -
H7F25_RS00910 226755..227243 + 489 WP_024425393.1 anti-sigma B factor RsbW -
H7F25_RS00915 227209..227997 + 789 WP_024425394.1 RNA polymerase sigma factor SigB -
H7F25_RS00920 227997..228596 + 600 WP_024425395.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8891

>T170087 WP_024425388.1 NZ_CP060191:223277-223627 [Bacillus sp. PAMC28571]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T170087 NZ_CP077081:698544-698906 [Pseudomonas atacamensis]
ATGGTGCGCCGACAGCCCCCTCAGCGGGGTGACGTTTACTGGATTGATCCAAACCCAGTGGCGGGGCGGGAGATGATGAA
CAGACATCGCTTTGTGGTGATCACGCCCAGAGAGATCAACGCATTGGGCGTGAGCATGACGGTGCCTGTTACTAGCGGTG
GGAATTTCAGCCGGAATATGGGTTTGGCGGTCGCCATTACTGACCACGAAACTAATGGTGTAGCGGTGTGTAATCAGGTT
CGCAGTTTTGACATAGAACAACGGGTACGAGACGGCACGGCCAAATTCATCGAGCGACTGGATGATGTGACAATGTCTGA
CATCGTCGACCGTGTAATCAGTGCAATCGACCCGCTAAGCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT170087 WP_003214273.1 NZ_CP060191:222991-223272 [Bacillus sp. PAMC28571]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT170087 NZ_CP077081:698275-698544 [Pseudomonas atacamensis]
ATGTCCGTAACTTCCAGGATACGCCGCCAAGGCGGCGCTGCCGTCATTACGATTCCGCCCACGCTTTTAAAGTTGTTGCA
TCTGGAGGTTGGCGCACAGCTAGAGCTGAATGTGGTCGATGGCGAACTGATTGCTCGTCCAGCCGTAGCCGCTACGCGTA
AGCGTTATAGTTTGTCAGAGCTACTCGAAGGCGCAGAAGAATTGCAGAAACTTAACGCCCAGACTAGCGAGACCCTTGAA
GGTGATTCTGTCGGCCGGGAGCTGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P7G713

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References