Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 1264..1865 Replicon plasmid p7
Accession NZ_CP060190
Organism Lactiplantibacillus plantarum strain Lp-6

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag H5V40_RS16915 Protein ID WP_001748110.1
Coordinates 1521..1865 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag H5V40_RS16910 Protein ID WP_001748109.1
Coordinates 1264..1527 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V40_RS16905 (H5V40_16740) 591..1178 - 588 WP_041153703.1 site-specific integrase -
H5V40_RS16910 (H5V40_16745) 1264..1527 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5V40_RS16915 (H5V40_16750) 1521..1865 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H5V40_RS16920 (H5V40_16755) 2448..2915 + 468 WP_042253884.1 DNA starvation/stationary phase protection protein -
H5V40_RS16925 (H5V40_16760) 2957..3046 - 90 Protein_4 IS30 family transposase -
H5V40_RS16930 (H5V40_16765) 3272..3766 + 495 WP_253734360.1 DUF536 domain-containing protein -
H5V40_RS16935 4043..4213 + 171 WP_162273789.1 hypothetical protein -
H5V40_RS16940 (H5V40_16770) 4624..5799 - 1176 Protein_7 IS256 family transposase -
H5V40_RS16945 (H5V40_16775) 5923..6357 + 435 WP_046041408.1 TM2 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..24406 24406


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T170086 WP_001748110.1 NZ_CP060190:1521-1865 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T170086 NZ_CP077081:c545732-545334 [Pseudomonas atacamensis]
ATGATCAAGTACATGCTGGATACAAATATCTGCATCTTCACCATCAAGAACAAACCACAAATTGTCCGGGAAGCCTTCAA
TCGACATTCCGGGCAGTTGTGCATCAGCGCTATCACGCTTATGGAGCTTATTTACGGTGCTGAAAAGTCGGCGGCCCCGG
AGAAAAATCTGGCAATTGTTGAAGGATTTGTCGCGCGACTGGATGTCTTGCCTTTCGATAACGACGCAGCCGCACAGGCC
GGGATGGTTCGATCTGAACTCGCGAAGGCTGGAACGCCGATCGGGCCATACGACCAAATGATCGCTGGCCACGCACGCTC
ACTTGGACTCATTGTAGTGACGAACAACGTACGGGAGTTTCAACGCGTTTCCGGTTTGCGAATCGAGGACTGGGTTTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT170086 WP_001748109.1 NZ_CP060190:1264-1527 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT170086 NZ_CP077081:c545962-545732 [Pseudomonas atacamensis]
ATGGAGCAGACCACAATTTTCATGAGCAACAGAAGTCAGGCAGTCCGACTGCCAAAGGCAGTTGCGATGCCCGCCGACGT
TAAACGAGTGGAAGTCATCGCGATTGGCAGAACACGCGTCATTACGCCTGCTGGAGAAAGCTGGGATAGCTGGTTTGATG
GCGACAGCGTCAGCCCTGATTTCATGGCTAACCGTGAGCAACCCACTGAACAGGAGCGCGAGTCGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References