Detailed information of TA system TA170085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 73193..73718
• Replicon: plasmid pSLT
• Accession: NZ_CP060170
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain BKQZM9

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: H7H28_RS24130
• Protein ID: WP_001159863.1
• Coordinates: 73193..73498 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: H7H28_RS24135
• Protein ID: WP_000813641.1
• Coordinates: 73500..73718 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7H28_RS24095	68579..69139	-	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-
H7H28_RS24100	69295..69582	+	288	WP_071530243.1	hypothetical protein	-
H7H28_RS24105	69859..70343	-	485	Protein_79	hypothetical protein	-
H7H28_RS24110	70847..71557	-	711	Protein_80	EAL domain-containing protein	-
H7H28_RS24115	71566..72348	-	783	WP_000082169.1	site-specific integrase	-
H7H28_RS24120	72383..72904	-	522	WP_000198608.1	hypothetical protein	-
H7H28_RS24125	72901..73191	-	291	WP_001266176.1	hypothetical protein	-
H7H28_RS24130	73193..73498	-	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
H7H28_RS24135	73500..73718	-	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H7H28_RS24140	74394..74915	+	522	WP_010999942.1	hypothetical protein	-
H7H28_RS24145	75399..75695	+	297	WP_001687482.1	hypothetical protein	-
H7H28_RS24150	76189..77178	+	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
H7H28_RS24155	77437..77881	-	445	Protein_89	site-specific integrase	-
H7H28_RS24160	78050..78431	-	382	Protein_90	YgiW/YdeI family stress tolerance OB fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93813	93813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 15030.03 Da
• Isoelectric Point: 5.5500

>T170085 WP_001159863.1 NZ_CP060170:c73498-73193 [Salmonella enterica subsp. enterica serovar Typhimurium]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 306 bp

>T170085 NZ_CP077080:4976425-4976640 [Pseudomonas alvandae]
GTGAATAGCCGATTCTTGATAAGCCAAACTGTTGCGGATGGTTGGTACCTGGTGCGTATCCGAGGCAGTCACCATCACTT
CAAGCACCCGACCAAACCGGGGCTGGTCACGGTTCCTCATCCCAAGAAGGACCTGCTCAAGAAGACGGCCATCAGTATCT
TGCAACAGGCCCTTCTCCAGACAGCCAGTTGCCCCGTTTTTCCGGAGGACGATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 10761.91 Da
• Isoelectric Point: 5.5500

>AT170085 WP_000813641.1 NZ_CP060170:c73718-73500 [Salmonella enterica subsp. enterica serovar Typhimurium]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 219 bp

>AT170085 NZ_CP077080:4976641-4977051 [Pseudomonas alvandae]
ATGCTTTACCCGATTGCGATTTCCATGGGCGATGACAAGCACGCCTGGGGTGTTGAGGTGCCGGATATTCCCGGTTGTTT
TTCCGCAGGTGACGACCTGGATGAGGCCATGGCGATGGCGCGTGAGGCGATCGAGGGGCATTTCGAGATTCTCGCTGAAG
ACGGCTCACCGATTCCATCGGCCAATACCGTTACCCTGCACGCGGCCGATCCGCGATATGCCGGGTGCACCTGGGCGCTA
GTGGATATTGATGTGACCAAATACCTGGGCAAGGCGCAGAAACTCAACATCACGTTGCCCGGCTACCTGCTCAACCGGAT
TGATGAATATGTGTTGAATCATCCTGAAGAGAAGAGCCGCTCCGGCTTCCTGGCATCGGCAGCGCTGAAGGTGCTGCAAC
AAGGGCGATGA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6