Detailed information of TA system TA170082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1013986..1014521
• Replicon: chromosome
• Accession: NZ_CP060160
• Organism: Staphylococcus lugdunensis strain MBAZ2

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: C5QLS6
• Locus tag: H6Y62_RS04705
• Protein ID: WP_002445368.1
• Coordinates: 1014198..1014521 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: C5QLS5
• Locus tag: H6Y62_RS04700
• Protein ID: WP_001058489.1
• Coordinates: 1013986..1014195 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H6Y62_RS04665	1009242..1009526	+	285	WP_002461410.1	co-chaperone GroES	-
H6Y62_RS04670	1009586..1011208	+	1623	WP_002461409.1	chaperonin GroEL	-
H6Y62_RS04675	1011277..1012449	-	1173	WP_002492309.1	site-specific integrase	-
H6Y62_RS04680	1012463..1013137	-	675	WP_002445345.1	helix-turn-helix transcriptional regulator	-
H6Y62_RS04685	1013310..1013528	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
H6Y62_RS04690	1013533..1013850	+	318	WP_002445364.1	helix-turn-helix domain-containing protein	-
H6Y62_RS04695	1013847..1013993	+	147	WP_002445365.1	hypothetical protein	-
H6Y62_RS04700	1013986..1014195	+	210	WP_001058489.1	hypothetical protein	Antitoxin
H6Y62_RS04705	1014198..1014521	+	324	WP_002445368.1	DUF1474 family protein	Toxin
H6Y62_RS04710	1014585..1015454	+	870	WP_002445370.1	primase alpha helix C-terminal domain-containing protein	-
H6Y62_RS04715	1015468..1017177	+	1710	WP_002445371.1	DUF927 domain-containing protein	-
H6Y62_RS04720	1017525..1017890	+	366	WP_002445372.1	hypothetical protein	-
H6Y62_RS04725	1017904..1018545	+	642	WP_002492310.1	pathogenicity island protein	-
H6Y62_RS04730	1018833..1019177	+	345	WP_002445374.1	hypothetical protein	-
H6Y62_RS04735	1019191..1019424	+	234	WP_002492317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1005578..1024659	19081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 13107.51 Da
• Isoelectric Point: 4.8571

>T170082 WP_002445368.1 NZ_CP060160:1014198-1014521 [Staphylococcus lugdunensis]
MNWEIKNLFSDLKLLKDSFEDLKDNHGWHFEELYPHEPNHNLNKDELIREGFSYHERRIHNNQMFDLFHLYIEQFDKIIQ
KFHELEKASSENFGEESDDAQKLKITE

Nucleotide

• Length: 324 bp

>T170082 NZ_CP077080:731563-731850 [Pseudomonas alvandae]
ATGACCGAACAATTCACTCGCTGGGACTCTGCCGAGTACCTCAAGACCGAAGAGGACATGGCGAACTATTTGGATGCATG
CATGGACGAGGCAGGAGATGACCCCGCATTCATTGCTAAAGCCCTAGGGACCATTGCACGAGCTCGTGGGATGACCCAGG
TGGCCCGTGACGCGGGCCTCTCTCGGGAAAACCTTTATCGCGCCTTGTCAGGGGAAGGAAACCCTGAGTTCGGTACTATT
TTGAAAGTTGTTAAAGCGTTGGGGCTGAAACTGCATGCCAGTACCTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8225.24 Da
• Isoelectric Point: 4.2820

>AT170082 WP_001058489.1 NZ_CP060160:1013986-1014195 [Staphylococcus lugdunensis]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT170082 NZ_CP077080:731261-731566 [Pseudomonas alvandae]
ATGACGGAGATCATTCGAAGCTCTACCTTTTCCGGCTGGCTCTCGAAGCTGGCTGATAGCCGTGCCAGGATGCGTATTCA
GGTTCGAGTCGATCGTATGGCCGATGGGAATCTGGGTGACGTCAAAGCTTTAGGAGAAGGGTTGAGTGAGGCCCGTATCG
ATTACGGTCCGGGCTATCGAATTTATTTCATGCAGCAAGGCCGACAATTGATCATCCTTCTGTGTGGTGGTGACAAAAGC
AGTCAAGCACGTGATATCAAGCAAGCCAGGCTGATCGCAAAATCCTGGCAGGAGCAAAACCCATGA

Structures

Toxin

• AlphaFold DB: A0A853V3H1

Antitoxin

• AlphaFold DB: A0A853V601