Detailed information of TA system TA170078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1553593..1554179
• Replicon: chromosome
• Accession: NZ_CP060159
• Organism: Xylella fastidiosa subsp. fastidiosa strain GV230

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q87BV5
• Locus tag: XFGV230_RS06945
• Protein ID: WP_004089517.1
• Coordinates: 1553925..1554179 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: XFGV230_RS06940
• Protein ID: WP_004085025.1
• Coordinates: 1553593..1553928 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFGV230_RS06910	1549192..1550046	-	855	WP_011098055.1	iron-sulfur cluster carrier protein ApbC	-
XFGV230_RS06915	1550538..1551311	+	774	WP_004089528.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
XFGV230_RS06920	1551308..1551772	+	465	WP_011098056.1	low molecular weight phosphotyrosine protein phosphatase	-
XFGV230_RS06925	1551759..1552472	-	714	WP_011098057.1	site-specific DNA-methyltransferase	-
XFGV230_RS06930	1552823..1553104	+	282	WP_011098058.1	type II toxin-antitoxin system RelE/ParE family toxin	-
XFGV230_RS06935	1553114..1553413	+	300	WP_004089519.1	HigA family addiction module antidote protein	-
XFGV230_RS06940	1553593..1553928	-	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
XFGV230_RS06945	1553925..1554179	-	255	WP_004089517.1	type II toxin-antitoxin system HicA family toxin	Toxin
XFGV230_RS06950	1554232..1554930	-	699	WP_011098059.1	hypothetical protein	-
XFGV230_RS06955	1555059..1555520	-	462	WP_011098060.1	hypothetical protein	-
XFGV230_RS06960	1555517..1556281	-	765	WP_004090689.1	hypothetical protein	-
XFGV230_RS06965	1556187..1557293	-	1107	WP_012382665.1	YdaU family protein	-
XFGV230_RS06970	1557290..1557589	-	300	WP_080513037.1	hypothetical protein	-
XFGV230_RS06975	1557588..1558065	+	478	Protein_1356	conjugal transfer protein	-
XFGV230_RS06980	1558071..1558676	+	606	WP_004091108.1	conjugal transfer protein TrbN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9639.14 Da
• Isoelectric Point: 11.2766

>T170078 WP_004089517.1 NZ_CP060159:c1554179-1553925 [Xylella fastidiosa subsp. fastidiosa]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T170078 NZ_CP077079:c1722590-1722408 [Pseudomonas asgharzadehiana]
GTGGATAGCCGATATTTGATAGGTCACATCGTTGCGGACGGTTGGTATCTGGTGCGCACAAGGGGCAGTCATCATCATTT
CAAACACCCTGTGAAGCCGGGGCTGGTGACGATTCCCCATCCAAAAAAGGACCTGTTGGACAAGACCGCCAAGAGCATTT
TGAAACAAGCTCTAATGAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT170078 WP_004085025.1 NZ_CP060159:c1553928-1553593 [Xylella fastidiosa subsp. fastidiosa]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT170078 NZ_CP077079:c1722385-1721975 [Pseudomonas asgharzadehiana]
ATGCTGTATCCCATCGCAATTTCAACGGGCGATAACGACCATGCCTGGGGTGTGGAAGTCCCGGATATTCCCGGCTGCTA
CTCAGCCGGCGACGACCTGGACGACGCCATGGCCATGGCCCGGGAAGCGATCGAAGGGCACTTCGAGATCCTCGCCGAAG
ACGGTGCACCGATTCCCAGCGCGCGCAAAGTCACCGTGCATGCCGCCAACCCGCAGTACGCCGGCTGCACATGGGCAGTC
GTGGATATCGACGTCACCAAGTACCTCGGCAAGGCGCAGAAACTCAACATCACCTTGCCGGGTTATCTGCTTAACCGCAT
AGATGAATACGTTCTGCACCACCCTGAAGAGAAAAGCCGTTCAGGTTTTCTCGCATCCGCGGCGCTGAAGGTGCTGCAAC
AGGATCGGTAG

Structures

Toxin

• AlphaFold DB: Q87BV5

Antitoxin

• AlphaFold DB: A0A060HAK5